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Tika RT-qPCR Kit II
◮Tika RT-qPCR Kit, ma te whakamahi i te Foreasy Reverse Transcriptase me te Foreasy HS Taq DNA Polymerase e whakawhanake ana e Foregene, me te aukati tauhohenga ahurei, ka hangaia tenei kete ki te kaha te aukati me te hototahi, a ka taea e ia te whakamatautau tika te whakamahi i te punaha Foregene Lysis hei tauira.Ka taea e tenei kete te tono mo te kete kimi waikawa nucleic COVID-19 me etahi atu huakita pathogenic ki te whanaketanga kete kitenga waikawa karihi.
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Tika RT-qPCR Kit III
◮Tika RT-qPCR Kit, ma te whakamahi i te Foreasy Reverse Transcriptase me te Foreasy HS Taq DNA Polymerase e whakawhanake ana e Foregene, me te aukati tauhohenga ahurei, ka hangaia tenei kete ki te kaha te aukati me te hototahi, a ka taea e ia te whakamatautau tika te whakamahi i te punaha Foregene Lysis hei tauira.Ka taea e tenei kete te tono mo te kete kimi waikawa nucleic COVID-19 me etahi atu huakita pathogenic ki te whanaketanga kete kitenga waikawa karihi.
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Tika RT-qPCR Kit
◮ Tika RT-qPCR Kit, ma te whakamahi i te Foreasy Reverse Transcriptase me te Foreasy HS Taq DNA Polymerase e whakawhanake ana e Foregene, me te aukati tauhohenga ahurei, ka hangaia tenei kete ki te kaha te aukati me te hototahi, a ka taea e ia te whakamatautau tika te whakamahi i te punaha Foregene Lysis hei tauira.Ka taea e tenei kete te tono mo te kete kimi waikawa nucleic COVID-19 me etahi atu huakita pathogenic ki te whanaketanga kete kitenga waikawa karihi.
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Tauira Kaituku Tuku
◮He ngawari ki te whakahaere:te lysis tauira i te wa e kohikohi ana i te tauira
◮He tere me te watea:Kaore he take mo te tangohanga waikawa karihi, e tika ana mo te kitenga tauine nui
◮Arahi tono whanui:PCR tika i muri i te kohinga tauira
◮Haumaru me te pono:Kia tere te whakakore i te huaketo, te whakarite i te haumaru ki te whānuitanga nui
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ForeDirect RT-qPCR Kit
◮Mo te RNA te whakakaha i te waa-a-waa mai i nga kohinga swab kaore he tukanga purenga RNA.Ka oti i tenei kete nga huringa qRT-PCR i roto i te haora kotahi.Ko te ranunga enzyme he whakakotahitanga papai o Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Kei roto i te Reaction Buffer nga waahanga katoa e hiahiatia ana, tae atu ki nga waahanga aukati kua arotauhia, Mg2+, dUTP, me nga dNTP.
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Foreasy M-MLV Whakamuri Transcriptase
Ko te Foreasy M-MLV Reverse Transcriptase he transcriptase whakamuri hou e whakaatuhia ana i roto i te huakita E. coli i hangaia ma te whakamahi i te hangarau whakakotahitanga ira.He DNA polymerase whakakotahi e whakahiato ana i te whenu DNA taapiri mai i te RNA, te DNA, te RNA:DNA ranu ranei.Karekau he mahi RNase H, he kaha te pumau, te hononga RNA kaha, me te aro nui ki te kitenga.
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Te aukati RNase Foreasy
◮He aukati RNase i ahu mai i te kiore hou e whakaatuhia ana i roto i nga huakita i hangaia e E. coli ma te whakamahi i te hangarau whakakotahi ira.Ka aukati te aukati i te mahi RNase ma te whakakotahi kore whakataetae me te RNase 1: 1, na reira ka tiakina.RNAte tapatahi, a ka taea te aukatiteRNase A, B, C mahi , engari ehara i te RNase T1, T2, H, me etahi atu; Ka taea te whakahuri o te here o RNase Inhibitor me RNase , a ka taea te wehewehe i te matatini e te urea me te sulfhydryl reagents, kia kore e taea te whakakore i te RNase renatures me te aukati.
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Foreasy HS Taq DNA Polymerase
◮Te tino motuhake: Ko te enzyme he nui te mahi wera wera.
◮Whakanuia Tere: 10 sec/kb.
◮Te urutau tauira teitei: ka taea te whakamahi kia pai te whakanui i te TeiteiGCuaraamomo tauira DNA uaua-ki-whakanui.
◮Te piripono kaha: Ko te pono e 6 nga waof noa Taq Enzyme.
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Foreasy Taq DNA Polymerase
◮Te tino motuhake: He mahi whakaoho wera to te enzyme.
◮Whakanuia Tere: 10 sec/kb.
◮Te tauira tino urutau: ka taea te whakamahi ki te whakanui i te GC Te uara teitei, nga momo tauira tauira DNA uaua-ki-whakanui.
◮Tino pono: Taq Enzyme 6 nga wa.
◮Te kaha o te waiariki: Ka taea te whakanoho ki te 37 °C mo te wiki me te pupuri i nga mahi neke atu i te 90%.
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