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Kete PCR Hiko Kiore Tika PCR Reagent Reagent Reagent PCR (Mouse Tail)(mo te Tohuaira)

Kiore Tail PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(mo te Tohua) Atahanga Whakaatu
  • Kete PCR Hiko Kiore Tika PCR Reagent Reagent Reagent PCR (Mouse Tail)(mo te Tohuaira)
  • Kete PCR Hiko Kiore Tika PCR Reagent Reagent Reagent PCR (Mouse Tail)(mo te Tohuaira)
  • Kete PCR Hiko Kiore Tika PCR Reagent Reagent Reagent PCR (Mouse Tail)(mo te Tohuaira)
  • Kete PCR Hiko Kiore Tika PCR Reagent Reagent Reagent PCR (Mouse Tail)(mo te Tohuaira)

Whakaahuatanga kete:

Whakamahi tika i te lysate hiku kiore hei tauira mo te whakakaha PCR, kaore he tangohanga motuhake o te DNA, he tere me te tairongo teitei.

◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

◮He iti te tono tauira, me tango he kakano kotahi.

◮Kaore e hiahiatia nga maimoatanga motuhake penei i te huri me te kuru, a he ngawari te mahi.

◮Ko te punaha PCR kua arotauhia ka taea e te PCR te whai kiko ake me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

te kaha o mua


Taipitopito Hua

Tohu Hua

Whakaahuatanga

Ka whakamahia e tenei kete he punaha buffer lysis motuhake hei tuku tere i te DNA ira mai i nga hiku kiore, nga taringa, nga uaua me etahi atu tauira kiko mo nga tauhohenga PCR, no reira he tino pai mo te whakamatautau ira-nui.

Ko te tukanga o te tuku i te DNA genomic mai i te lysis buffer ka oti i roto i te 10-30 meneti i te 65°C.Karekau he tukanga pera i te tango i te pūmua me te RNA e hiahiatia ana, ka taea te whakamahi te tohu DNA hei tauira mo te tauhohenga PCR.

2× M1-PCR HewariTMHe kaha te manawanui o te ranunga ki nga aukati tauhohenga PCR, a ka taea te whakamahi i te lysate o te tauira hei whakamatautau hei tauira mo te whakakaha pai me te motuhake.Kei roto i tenei reagent Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, tauhohenga buffer, PCR optimizer and stabilizer.

Ko te D-Taq DNA polymerase he DNA polymerase i hangaia e Foregene mo nga tauhohenga PCR tika.Ko te D-Taq DNA polymerase he kaha te manawanui ki nga momo aukati tauhohenga PCR, a ka taea e ia te whakanui i nga tohu o te DNA i roto i nga momo punaha tauhohenga uaua, a ka eke te tere whakakaha ki te 2Kb/min, he mea tino pai mo te tauhohenga PCR Tika.

 

Whakatakotoranga

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nga waahanga kete

Wāhanga I

Pupuri MP

Foregene Protease Plus

6× DNA Loading Buffer

Wahi II

2× M1 PCR NgawariTMWhakaranu

Tohutohu

Nga waahanga me nga painga

■Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

■ He iti te tono tauira, ka taea te whakamatautau i te 5mg kiko kiore, 2-5mm ranei te hiku kiore.

■Kaore e hiahiatia nga maimoatanga motuhake penei i te huri me te kuru, a he ngawari te mahi.

■ Ko te punaha PCR kua arotauhia ka nui ake te motuhake o te PCR me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

Tawhā kete

Te tono: Te hiku kiore, te taringa kiore, te uaua me etahi atu kopa.

Ko te DNA i tukuna mai i te tauira lysis: whakamahia anake hei tauira PCR.

Ka taea te whakamahi i te kete mo nga kaupapa e whai ake nei: te tautuhi i te transgenosis, te whakapapa kararehe, aha atu.

Te rere mahi

wk flw-kiore hiku tika PCR

Rokiroki me te ora o te Papa

Ko te wahanga I o tenei kete me penapena ki te 2-8℃.

Ko te reagent Buffer AL ka taea te penapena mo nga marama 12 i raro i nga waahi maroke;ka taea te penapena i te 2-8℃mo te rokiroki roa.

 He tātai ahurei tā Reagent Foregene Protease.Hei whakarite i tana mahi me te pumau, tiakina ki te 4℃mo te 12 marama.

 Reagent 6×Ka taea te penapena DNA Loading Buffer ki te 4℃ranei -20℃mo te wa roa.

Ko te wahanga II o tenei kete me pupuri ki te -20℃.

 Reagent 2×M1 PCR ngawariTMWhakaranuhia, mena ka whakamahia i nga wa maha, ka taea hoki te rongoa ki te 4℃mo te rokiroki mo te wa poto (whakamahia i roto i nga ra 10).

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