China Kiore Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(mo te Genotyping) Kaihanga me te Kaiwhakarato |Foregene

Kete PCR Tika Hiko Kiore-UNG Direct PCR Reagent Lysis Reagent(Tail Kiore)(mo te Tohua-ira)

Kete PCR Tikanga Hiko Kiore-UNG Direct PCR Lysis Reagent(Tail Kiore)(mo te Whakatauira) Atahanga Whakaatu

Whakaahuatanga kete:

Ka whakamahia tika te lysate hiku kiore hei tauira mo te whakanui PCR me te kore tangohanga motuhake o te DNA, he tere me te tino tairongo.Kua taapirihia te punaha anti-parahanga UNG ki te whakakore i nga hua pai.

◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

◮He iti te tono tauira, me tango he kakano kotahi.

◮Kaore e hiahiatia nga maimoatanga motuhake penei i te huri me te kuru, a he ngawari te mahi.

◮Ko te punaha PCR kua arotauhia ka taea e te PCR te whai kiko ake me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

Tukuna he imeera ki a matou

Taipitopito Hua

Tohu Hua

Whakaahuatanga

Ka whakamahia e tenei kete he punaha buffer lysis motuhake hei tuku tere i te DNA ira mai i nga hiku kiore, nga taringa, nga uaua me etahi atu tauira kiko mo nga tauhohenga PCR, no reira he tino pai mo te whakamatautau ira-nui.

Ko te tukanga o te tuku i te DNA genomic mai i te lysis buffer ka oti i roto i te 10-30 meneti i te 65°C.Karekau he tukanga pera i te tango i te pūmua me te RNA e hiahiatia ana, ka taea te whakamahi te tohu DNA hei tauira mo te tauhohenga PCR.

2× M1-PCR Hewari^TMKa whakamahia e Mix (UNG) te dUTP hei utu mo te dTTP i runga i te 2× M1-PCR Easy^TMWhakaranuhia (UNG), ka taapirihia te UNG enzyme (Uracil-N-glycosylase) ka taea te whakaheke i te tauira kei roto te dUTP i te wa ano.I mua i te tauhohenga PCR, ka whakamahia te enzyme UNG ki te whakaheke i te hua PCR kei roto te uracil.Karekau he paanga o te UNG enzyme ki te tauira karekau he uracil, na reira ka whakarite i te motuhake me te tika o te whakakaha me te aukati i te tupono o te whakamatautau ira-nui.Kua pokea nga hua PCR.

Ko te D-Taq DNA polymerase he DNA polymerase i hangaia e Foregene mo nga tauhohenga PCR tika.Ko te D-Taq DNA polymerase he kaha te manawanui ki nga momo aukati tauhohenga PCR, a ka taea e ia te whakanui i nga tohu o te DNA i roto i nga momo punaha tauhohenga uaua, a ka eke te tere whakakaha ki te 2Kb/min, he mea tino pai mo te tauhohenga PCR Tika.

Whakatakotoranga

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nga waahanga kete

Wāhanga I	Pupuri MP
	Foregene Protease Plus
	6× DNA Loading Buffer
Wahi II	2× M1 PCR Ngawari^TMWhakaranu(UNG)
Tohutohu

Nga waahanga me nga painga

■Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

■ He iti te tono tauira, ka taea te whakamatautau i te 5mg kiko kiore, 2-5mm ranei te hiku kiore.

■ Kaore e hiahiatia nga maimoatanga motuhake penei i te huri me te kuru, a he ngawari te mahi.

■ Ko te punaha PCR kua arotauhia ka nui ake te motuhake o te PCR me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

■ Pūnaha PCR anti-parahanga 2×M1-PCR ngawari^TMWhakaranu (UNG), e whakakore pai ana i te parahanga na nga hua PCR me te whakarite i te motuhake me te tika o te whakakaha.

Tawhā kete

Te whānuitanga o te tono: te hiku kiore, te taringa kiore, te uaua me etahi atu kopa.

Ko te DNA i tukuna mai i te tauira lysis: whakamahia anake hei tauira PCR.

Ka taea te whakamahi i te kete mo nga kaupapa e whai ake nei: te tautuhi i te transgenosis, te whakapapa kararehe, aha atu.

Te rere mahi

Rokiroki me te ora o te Papa

Ko te waahanga tuatahi o tenei kete me penapena ki te 2-8 ℃.

Ko te reagent Buffer AL ka taea te penapena mo nga marama 12 i raro i nga waahi maroke;ka taea te penapena ki te 2-8 ℃ mo te rokiroki roa.

He tātai ahurei tā Reagent Foregene Protease.Hei whakarite i tana mahi me te pumau, tiakina ki te 4 ℃ mo te 12 marama.

Reagent 6×DNA Loading Buffer ka taea te penapena i te 4 ℃, -20 ℃ ranei mo te wa roa.

Ko te wahanga II o tenei kete me pupuri ki te -20 ℃.

Reagent 2×M1 PCR EasyTM Mix(UNG), ki te whakamahia auau, ka taea hoki te penapena ki te 4 ℃ mo te rokiroki mo te wa poto (whakamahia i roto i nga ra 10).

Tōmua: Te Hiko Kiore Kete PCR Tupato Tuturu PCR Reagent Raihana(Tae Kiore)(mo te Tohuaira)

Panuku: Kete PCR Hiko Kiore Tika PCR Reagent Reagent Reagent PCR (Mouse Tail)(mo te Tohuaira)

Tuhia to korero ki konei ka tukuna mai ki a matou