Haina Kiore Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(mo te Genotyping) Kaihanga me te Kaiwhakarato |Foregene

Te Hiko Kiore Kete PCR Tupato Tuturu PCR Reagent Raihana(Tae Kiore)(mo te Tohuaira)

Tail Kiore SuperDirect PCR Kit Direct PCR Lysis Reagent(Tail Kiore)(mo te Whakatauira) Atahanga Whakaatu

Whakaahuatanga kete:

Whakamahi tika i te lysate hiku kiore hei tauira mo te whakakaha PCR, kaore he tangohanga motuhake o te DNA, he tere me te tairongo teitei.

◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

◮He iti te tono tauira, me tango he kakano kotahi.

◮Kaore e hiahiatia nga maimoatanga motuhake penei i te huri me te kuru, a he ngawari te mahi.

◮Ko te punaha PCR kua arotauhia ka taea e te PCR te whai kiko ake me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

Tukuna he imeera ki a matou

Taipitopito Hua

Tohu Hua

Whakatakotoranga

Ka whakamahia e tenei kete he punaha putunga lysis motuhake hei tuku tere i te DNA ira mai i te hiku kiore me nga tauira taringa kiore mo te tauhohenga PCR, no reira he tino pai mo te whakamatautau ira-nui.

Kua oti te tukanga o te tuku i te DNA genomic mai i te lysis bufferi roto i te 10-30 menetiite pāmahana rūma (20-25°C).Kaore e hiahiatia he whakamahana, kaore e hiahiatia etahi atu tukanga penei i te pūmua me te tango RNA.Ka taea te whakamahi i nga waahanga DNA hei tauira mo te tauhohenga PCR.

2×M-PCR Hewari^TMHe kaha te manawanui o te ranunga ki nga aukati tauhohenga PCR, a ka taea te whakamahi i te lysate o te tauira hei whakamatautau hei tauira mo te whakakaha pai me te motuhake.Kei roto i tenei reagent Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, tauhohenga buffer, PCR optimizer and stabilizer.

Ko te D-Taq DNA polymerase he DNA polymerase i hangaia e Foregene mo nga tauhohenga PCR tika.Ko te D-Taq DNA polymerase he kaha te manawanui ki nga momo aukati tauhohenga PCR, a ka taea e ia te whakanui i nga tohu o te DNA i roto i nga momo punaha tauhohenga uaua, a ka eke te tere whakakaha ki te 2Kb/min, he mea tino pai mo te tauhohenga PCR Tika.

Whakatakotoranga

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nga waahanga kete

Wāhanga I	Kaipupuri NP
	Foregene Protease Plus I
	6× DNA Loading Buffer
Wahi II	2× M PCR EasyTM Mix
Tohutohu

Nga waahanga me nga painga

■Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

■ He iti te tono tauira, tangohia noa nga hiku kiore (he taringa kiore ranei me te diameter o te 2-5mm) kei runga ake i te 2mm.

■ He ngawari ki te whakahaere, ka taea te lysed me te kore whakamahana ki te whakakore i te protease, a ka taea te tuku tika i te hua lysed ki te tauhohenga PCR.

■ Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) ka taea te whakamahi ki te tango i te DNA ira tangata mai i te hiku kiore kua tukatukahia.

■ Hangaia e te punaha PCR kua arotauhia te ahua o te PCR me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.