W: E te tuakana, kua oti te whakamaoritanga whakamuri, ka mutu ko te mahi whakamutunga – te PCR ine ine kareoro!Kia ora, e te tuakana, he aha te PCR ine-wa-tuuturu?
M: PCR kei te mohio koe i nga wa katoa, tika?
W: E mohio ana koe, ko te PCR te tauhohenga mekameka polymerase, a he hangarau e whakanui ana i te DNA!
M: Kua tata… He tikanga hangarau ki te whakanui ake i te raupapa whainga ki te neke atu i te kotahi miriona taima na roto i nga tohu tuatahi, engari he tohu noa tenei i te noho, i te ngaro ranei o te raupapa whaainga, kaore e taea te whakatau i te nui o te tauira tuatahi.Ko te PCR ine ine korikori he tikanga hangarau i hangaia hei whakaoti i tenei raru.Ko te PCR mo te wa tuturu me te PCR rahinga (qPCR) e korero ana.
W: Ko te rereketanga mai i te PCR noa kei roto i te ine ine korakora?
M: Ae, ara, i roto i te wahanga toronga o ia huringa PCR, ka kitea te kaha o te tohu ororongo, a ka taea te tatau i te nui o te tauira tuatahi i runga i te huringa o te kaha tohu.
W: He tino uaua!Me tatau ake e au tenei?
M: Ko tenei me ata ako koe, he pai ake kia mohio koe ki te kaupapa.Inaianei ka taea e koe te aro ki te uara CT i te tuatahi, he raraunga whakamohio ngawari, me tona rahi e rite ana ki te rahi o te rahinga tauira tuatahi.He kauwhata ano pera i nga pihi whakakaha me nga pihi whakarewa.Kei a ratou te korero whakamutunga mo te angitu o to whakamatautau, kaore ranei.
W: Aue, teina.Na ka mahi ahau i te whakamatautau qPCR!
M: Kia tupato kia kaua e taapiri i nga tauira ki te waahi reagent, ki te kore ka pokea to ranunga kaore i whakamahia!
W: Kua riro!

Wā Tuturu PCR Easyᵀᴹ-SYBR Green I Kit

He pai ake te kaha, he nui ake te motuhake
Te tairongo teitei, te hototahi pai