Kia ora, e hoa ma!
I muri i te mahi tangohanga waikawa nucleic mo nga tau maha, kei te tino mohio te katoa ko te kounga o te tangohanga waikawa karihi te mea matua mo te angitu me te tika o nga whakamatautau ka whai ake.
He maha nga wa, kei te maataki noa tatou mehemea kei roto te owehenga o OD260/OD280 i roto i te awhe tino pai.Ka rite ki tenei…
E ai ki te whakatauki, kare koe e kaha ki te korero, kare hoki e koa moata.
Tuatahi whakarongo ki te Etita e korero ana ki a koe he korero iti!
Ko enei korero pakiwaitara ataahua ka tupu i roto i nga taiwhanga e mahi tangohanga waikawa karihi.
I whakaaro koe ko RNA anake koe i tango, Aroha mai!
Inaa, ko te mea kaore koe e mohio kei te huna huna a Mr Protein.
Ki to whakaaro kei te pirangi koe kia puta a Mr Protein me te OD260/OD280 anake?
He tamariki rawa!He ngawari rawa!
Me whakamahi e matou te tikanga whakapouri hiriwa tairongo hei awhina i a koe ki te kite i nga pūmua huna i roto i te RNA!
| Hua whakamahia | OD260/280 | Mtaputapu whakaata |
| Foregene | 2.09 | Thermo ScientificTMNanoDropTMKotahi Ultra-trace UV-Vis Spectrophotometer |
| Trizol | 1.91 |
I nga wa o mua, he hoa piri te pūmua me te RNA.
He whakama ake a Mr Protein.
Na ko te RNA kaha me te koa ki te takaro ki a ia.
Kotahi ra.
I kii a RNA ki a Mr Protein ka tangohia e etahi atu, a ka tino pouri a Mr Protein.
Ko te RNA hoki e tino pukuriri ana ki a Mr pūmua, a ka kii: "Me pehea taku peera atu i a koe, ka huna koe i roto i oku kakahu, ka ngana kia kaua e kitea e etahi, pai?"
Ka ataata a Mr. Protein me te whakaae koa.
I te ra o te whakatamarikitanga, ka huna a Mr Protein ki roto i nga kakahu o RNA, kaore i kitea.I whai ia i te tangata whangai i te hokinga mai ki te kainga, ka noho harikoa mai ano.
Te whakataurite o te tangohanga RNA me te ForegenePūtauTapekeKete Tangohanga RNA
me te Trizol Reagent a Kamupene T
Ka tino kite tatou i te paihere i te ara 4 (Trizol), ko te huarahi 3 (Foregene) he ahua ma.I whakapumautia e nga hua ko nga poke o te pūmua RNA i tangohia e te tikanga Trizol he maha tonu nga pata!A ko te ara 3 (Foregene) tata kore he poke pūmua na te mea i whakamahia e te kaiwhakamatautau te kete tangohanga RNA Foregene!(I tae ohorere mai te panui)
He korero ano:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Wā tuku: Feb-10-2022
