Neke atu i te haurua o te tau 2022, haere mai kia kite he aha nga tuhinga tohu teitei kua tukuna e Foeregene!

01

Mena: 17.694

I taia e: Te Hospital Hononga Tuatahi, Te Whare Wananga o Sun Yat-sen

Ko te ihirangi matua: Ko te mate pukupuku o te esophageal squamous cell carcinoma (ESCC) te momo iti o te mate pukupuku esophageal.Ko te tautuhi i nga whaainga rongoa whai hua he mea nui kia pai ake te maimoatanga o te ESCC.Kua whakaatuhia e etahi rangahau ka taea e te RNA mismodification te whakatairanga i te korero me te whakamaoritanga o nga mRNA e pa ana ki nga pūtau mate pukupuku, engari kei te noho marama tonu te tikanga ngota.I whakaatuhia e tenei rangahau ko te tRNA m7G methyltransferase-related complex proteins METTL1 me WDR4 i tino piki ake i roto i nga kopa ESCC me te whai waahi ki te mate kino o te ESCC.Mai i tera wa, ko te METTL1 me te WDR4 e pa ana ki nga rangahau ira ira i roto i nga kiore tauira ESCC kua whakapumautia ko te mahi whakarei ake o te tRNA m7G methyltransferase e arai ana ki te whakatikatika i te whakaaturanga o enei pūmua e rua, e whakatairanga ana i te tipu o nga pūtau ESCC.Ko tenei rangahau e whakaatu ana i tetahi mahi oncogenic nui o te whakarereketanga tRNA m7G i roto i te ESCC me te kii ko te whai i te METTL1 me ona huarahi tohu o raro kei te oati i nga whaainga rongoa mo te rongoa ESCC.

Hua awhina:

Kiore Tail Direct PCR Kit Foregene#TP-01333

Hononga taketake:

https://www.nature.com/articles/s41467-022-29125-7

02

Mena: 14.903

I whakaputahia e: Te Whare Taiwhanga Matua o te Rangahau Kounga i roto i te rongoa Hainamana, Institute of Chinese Medical Sciences, University of Macau

Ihirangi matua:

Na te whanaketanga o te hangarau waihanga, kua riro te mRNA hei karaehe hou mo nga rongoa rongoa.Heoi, he uaua tonu te whakatutuki i te tuku intracellular pai o nga raau taero mRNA na te kaha o te mRNA ki nga RNases.Ka whakaarohia e tenei rangahau he kaupapa tuku pai o nga hanganga nano-polyphenol-conjugated ka taea te tuku i te IL-10 mRNA i roto i te vivo.I muri i te whakamahi i tenei nanostructure ki te tuku i nga raau taero mRNA, ko nga taumata IL-10 i tino whakatikatikahia, he pai te aukati i te whakapuakitanga o nga mea mumura, te whakatairanga i te whakatikatika mucosal, te tiaki i nga pūtau epithelial colonic mai i te apoptosis, me te aukati i te dextran sodium sulfate-induced acute and chronic iti Ko te tauira murine colitis he nui nga hua whakaora, e whakarato ana i te hoahoa mate pukupuku ira hou.

Hua awhina:

Kararehe Tapeke RNA Isolation Kit Foregene#RE-03011

Hononga taketake:

https://www.sciencedirect.com/science/article/pii/S2211383522001551#:~:text=The%20polyphenol-assisted%20IL-10%20mRNA%20delivery%20system%20with%20self-protection,dextran%20sulfate%20tsolitis%20sulfate%20sulfate%20s tauira.%202.

03

Mena: 12.499

I whakaputahia e: Te Whare Taiwhanga o te Tauira Mate Kararehe Whakamatau, Te Kareti o te rongoa kararehe, Te Whare Wananga Ahuwhenua o Sichuan

Ihirangi matua:

Ko te ahotea oxidative o te parāoa he take nui e arai ana ki te kore tamariki o te tane, no reira he huarahi whaihua me te whai hua ki te whakatu i nga tauira kararehe kore tamariki ma te whakatikatika i te punaha antioxidant i runga i nga tikanga kua whakaritea.Heoi ano, kaore ano kia whakamaaramahia nga tikanga antioxidant motuhake mo nga pūtau iroriki tane.I whakapumautia e tenei rangahau ko te ira LanCL1 he ira antioxidant motuhake i roto i nga pūtau ira tangata, ka whakahekehia, ka hono ranei ki te kore tamariki o te tane.Ko nga kiore tane he kore i roto i te ira LanCL1 e whakaatu ana i te kino o te waikura me te ngoikore o te hua.I roto i te tukanga o te parāoa pārōnakitanga o ngā pūtau iroriki, ina puta te ahua o te hāoratanga, ko te pūmua 1 (SP1) te whakamaoritanga tauwehe-motuhake ka whakahaere i te whakapuakitanga o te ira LanCL1, na reira ka aukati i te tukanga hāoratanga o te parāoa.Ko tenei rangahau e whakaatu ana ko te huarahi SP1-LanCL1 he mahi nui ki te whakahaere i te testicular homeostasis me te hua o te tane i takawaenga e te toenga redox, me te kii he tono ataahua nga kiore kua whakarerekehia e LanCL1 hei tauira kararehe mo te kore tamariki.

Hua awhina:

Kiore Tail DNA Mini Kit Foregene#DE-05211

Hononga Taketake:

http://europepmc.org/article/MED/35469022

Kiore Tail Direct PCR Kit

◮Karekau he purenga DNA e pau ana te wa me te utu nui.

◮He iti noa te tono tauira, tangohia te kakano kotahi.

◮Karekau he maimoatanga motuhake penei i te huri me te kuru, he ngawari te mahi.

◮Ko te punaha PCR kua arotauhia ka taea e te PCR te whai kiko ake me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

Kararehe Tapeke RNA Isolation Kit

◮Kaore e tika ki te awangawanga mo te whakahekenga o te RNA.Ko te punaha katoa he RNase-Kore.

◮Te tango i te DNA ma te whakamahi i te Tiwae Whakapai DNA.

◮Tangohia te DNA me te kore e taapiri i te DNase.

◮Maama—ka oti nga mahi katoa i te pāmahana rūma.

◮Kare—ka oti te mahi i roto i te 30 meneti.

◮Haumaru—kaore he reagent waro i whakamahia.

◮Te parakore—OD260/280≈1.8-2.1.

Kiore Tail DNA Mini Kit

◮Karekau he poke RNase:Ko te Tiwae DNA-anake e whakaratohia ana e te kete ka taea te tango i te RNA mai i te DNA genomic me te kore e taapiri i te RNase i te wa o te whakamatautau, kia kore ai e poke te taiwhanga e te RNase o waho.

◮Tere tere:He nui ake te mahi a Foregene Protease i nga momo porotease rite, a ka keri tere i nga tauira kiko;he ngawari te mahi, a ka taea te mahi tangohanga DNA genomic i roto i nga meneti 20-80.

◮Watea:Ka mahia te centrifugation i te pāmahana rūma, a kaore he hiahia mo te 4°C iti-mahana centrifugation me te waihanol precipitation o DNA.

◮Haumaru:Kaore he tangohanga reagent waro e hiahiatia ana.

◮Kounga teitei:Ko te DNA genomic kua tangohia he mahanga nui, kahore he RNA, kahore he RNase, he iti rawa te ihirangi katote, ka taea te whakatutuki i nga whakaritenga o nga momo whakamatautau.

◮Pūnaha moroiti:Ka taea e ia te whakanui ake i te kukū o te DNA genomic, he watea mo te rapunga o raro, te whakamatautau ranei.