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PCR Heroᵀᴹ
◮Te pono teitei ake: 6he taima ki tera o Taq enzyme noa;
◮Tere ake atere whakaemi:ko te tere whakakaha he 5-10sec/kb, he 6-12 nga wa o te Taq enzyme noa;
◮He nui ake te urutau tauira: ka taea e ia te whakanui ake i nga momo tauira DNA matatini me nga ihirangi GC teitei me te uaua ki te whakanui;
◮Teitei akeTe kaha whakakaha: he iti ake te maha o nga huringa whakakaha ake i tera o Taq enzyme noa;
◮Staratara Te manawanui o te taiao: ka whakanohoia ki te 37°C mo te wiki, kia nui ake te pupuri90%mahi;
◮He 5'→3' te mahi polymerase DNA me te 5'→3' mahi exonuclease, kaore he mahi exonuclease 3'→5'.
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ī-mēra
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