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Wā Tuturu PCR Easyᵀᴹ-SYBR Green I kete
Maama—2X PCR Mix hei whakaiti i te hapa whakamatautau me te wa mahi
Ka taea e te parepare kua tino arotautia me te wera-whana Taq enzyme te aukati i te whakakaha kore-motuhake me te hanga dimer tuatahi
Te tairongo teitei—ka kitea nga kape iti o te tauira
He pai te mahi—he hototahi ki te nuinga o nga taputapu PCR ine i te waa-tūturu
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RT-PCR Easyᵀᴹ I(Kotahi Hipanga)
◮Ma te kete kotahi-taahiraa ka taea te whakawhiti whakamuri me te PCR ki te ngongo kotahi.Me taapiri noa i te tauira RNA, nga tohu tuatahi PCR me te RNase-Free ddH2O.
◮Ka taea te whakahaere tere me te tika te tātari ine ine o te RNA.
◮Ka whakamahia e te kete he reagent whakawhiti whakamuri motuhake a Foregene me te Foregene HotStar Taq DNA Polymerase i honoa me te punaha tauhohenga ahurei kia pai ake ai te kaha whakakaha me te motuhake o te tauhohenga.
◮Ko te punaha tauhohenga kua arotauhia ka nui ake te maaramatanga o te tauhohenga, te kaha ake o te waiariki, me te pai ake o te manawanui.
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RT-PCR Easyᵀᴹ II(E Rua Hipanga)
◮Ka taea te whakahaere tere me te tika te tātari ine ine o te RNA.
◮Ka whakamahia e te kete he reagent whakawhiti whakamuri motuhake a Foregene me te Foregene HotStar Taq DNA Polymerase i honoa me te punaha tauhohenga ahurei kia pai ake ai te kaha whakakaha me te motuhake o te tauhohenga.
◮ Ko te punaha tauhohenga kua arotauhia ka nui ake te maaramatanga o te tauhohenga, te kaha ake o te waiariki, me te pai ake o te manawanui.
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Wā Tuturu PCR Easyᵀᴹ-Taqman
◮Maama—2× PCR Mix hei whakaiti i te hapa whakamatautau me te wa mahi
◮Ka taea e te parepare kua tino arotautia me te wera-whana Taq enzyme te aukati i te whakakaha kore-motuhake me te hanga dimer tuatahi
◮Te tairongo teitei—ka kitea nga kape iti o te tauira
◮He pai te mahi—he hototahi ki te nuinga o nga taputapu PCR ine i te waa-tūturu
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PCR Heroᵀᴹ (Me te Waikano)
◮Te pono teitei ake: e 6 nga wa i to Taq enzyme noa;
◮Tere whakakaha tere
◮He nui ake te urutau tauira
◮He pai ake te whakakaha ake
◮He kaha ake te manawanui o te taiao: ka whakanohoia ki te 37 ° C mo te wiki, me te pupuri i nga mahi neke atu i te 90%;
◮He 5'→3' te mahi polymerase DNA me te 5'→3' mahi exonuclease, kaore he mahi exonuclease 3'→5'.
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700bp te arawhata DNA
◮Ko te 700bp DNA Ladder he hua kua reri ki te whakamahi, kei roto 1x Uta Puawai.Ka taea e koe te tango tika i te nui o te Tohu mo te electrophoresis i runga i nga hiahia whakamatautau.
700bpKo te Tohu DNA Ⅰ e 7 nga roopu DNA, ko nga roopu DNA ko: 100bp (50ng/5μl), 200bp (30ng/5μl), 300bp (30ng/5μl), 400bp (40ng/5μl), 500bp (25ng/5μl), 500bp (25ng/5μl), 30μl (25ng/5μl), 300bp (25ng/5μl) 5ng/5μl).
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2000bp te arawhata DNA
◮ Ko te 2000bp DNA Ladder he hua kua reri ki te whakamahi, kei roto 1x Uta Buffer.Ka taea e koe te tango tika i te nui o te Tohu mo te electrophoresis i runga i nga hiahia whakamatautau.Ko te Tohu DNA BM2000 e 6 nga roopu DNA, ko nga roopu DNA: 100bp (50ng/5μl), 250bp (50ng/5μl), 500bp (50ng/5μl), 750bp (75ng/5μl), 1000bp (50ng/5μl), 1000bp (50ng/5μl), 1000bp (50ng/5μl), 1000bp (50ng/5μl).
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5000bp te arawhata DNA
◮ 2000bp te arawhata DNAhe hua kua reri-ki-te whakamahi me te 1x Uta Buffer kei roto.Ka taea e koe te tango tika i te nui o te Tohu mo te electrophoresis i runga i nga hiahia whakamatautau.Ko te BM5000 DNA Marker e 8 nga roopu DNA, ko nga roopu DNA ko: 100bp(50ng/5μl), 250bp(50ng/5μl), 500bp(50ng/5μl), 750bp(75ng/5μl), 1000bp(50μl), 1000bp(50μl), 5μl(50μl), 1000bp(50μl),/5μl 00bp(30ng/5μl), 5000bp(50ng/5μl).
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PCR Easyᵀᴹ (me te tae)
Ko te 2 × PCR HeroTM He teitei ake te manawanui o te punaha whakaranu ki nga aukati PCR i te punaha PCR Mix noa, a ka ngawari ki te whakatutuki i te whakakaha PCR o nga momo tauira uaua.Ko te punaha tauhohenga ahurei me te kaha nui o Taq Hero ka nui ake te kaha o te whakakaha, te motuhake me te tairongo o te tauhohenga PCR.
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PCR Easy
Ko te punaha tauhohenga ahurei me te kaha nui o te Taq DNA Polymerase ka nui ake te pai o te whakakaha, te motuhake me te tairongo o te tauhohenga PCR.
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PCR Heroᵀᴹ
◮Te pono teitei ake: 6he taima ki tera o Taq enzyme noa;
◮Tere ake atere whakaemi:ko te tere whakakaha he 5-10sec/kb, he 6-12 nga wa o te Taq enzyme noa;
◮He nui ake te urutau tauira: ka taea e ia te whakanui ake i nga momo tauira DNA matatini me nga ihirangi GC teitei me te uaua ki te whakanui;
◮Teitei akeTe kaha whakakaha: he iti ake te maha o nga huringa whakakaha ake i tera o Taq enzyme noa;
◮Staratara Te manawanui o te taiao: ka whakanohoia ki te 37°C mo te wiki, kia nui ake te pupuri90%mahi;
◮He 5'→3' te mahi polymerase DNA me te 5'→3' mahi exonuclease, kaore he mahi exonuclease 3'→5'.
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QuickEasyᵀᴹ Wā Tūturu PCR Kit-Taqman
◮Ko te punaha arotautanga PCR ahurei ka hanga 2×QuickEasyTMTino PCR Mix-Taqman he hototahi ake.
◮Ko te Hot-start Foregene HS Taq Polymerase he pai ake te kaha whakakaha, teitei ake te tairongo whakakaha, me te motuhake whakakaha ake.
◮2× TereTMReal PCR Mix -Ka whakamahi a Taqman i tetahi punaha ahurei i arotauhia e Foregene ki te whakapai ake i te maaramatanga me te motuhake o te rapunga rangahau-a-raupapa, ka taea te whakamahi mo te taatai me te whakatau i nga rereketanga tau kape me te whiwhi hua tika.
◮Ko tenei hua ka tae mai me te tae tohutoro o roto o te ROX, ka taea te whakamahi hei whakakore i te papamuri tohu me te hapa tohu i waenga i nga puna, he pai mo nga kaihoko ki te whakamahi i nga momo taputapu PCR ine.
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