Kei te whakamahia nuitia a Taq enzyme hot-start.Ka whakatauritea ki te polymerase DNA noa, ka taea e te wera-tīmatanga Taq enzyme te karo i etahi whakakaha kore-motuhake me te hanganga o nga dimer tuatahi, a ka taea te whakapai ake i te reiti angitu o te whakakaha ira ira.Ina koa i roto i te waahi o te whakamatautau ira, kua tohuhia te wera-tīmatanga Taq enzyme hei paerewa mana i roto i te umanga, a kaua e whakamahia te polymerase DNA noa.Ka kitea mai i runga ake nei, ka whakamahia nuitia nga whaariki Taq wera-wera.I tenei wa, he maha nga tohu o te wera-tīmatanga Taq enzymes i runga i te maakete o-whare, engari kaore i te maha nga tohu wera-start Taq enzymes me te kounga teitei.Ka pa ana ki te maha o nga hua o te whaariki Taq, me pehea e whiriwhiri ai?

1. Tīpakohia te wera-tīmatanga Taq whākōkī me te kaha whakakaha teitei

Ko te kaha o te whakakaha PCR e hono tata ana ki te mahi a Taq enzyme.Whai muri i te pai o te punaha tauhohenga a Taq, ko te kaha o te whakakaha kei runga ake i te 95%, a he whanui te awhe whakakaha o te nui tauira tuatahi.Ka taea te whakakaha pai i te wa e iti ana te ihirangi ira ira, a kare i te ngawari ki te paihana ina he nui te rahi o te tauira, me te roa o te waa whakakaha.Mo te enzyme Taq he ngoikore te mahi, ahakoa he maha nga wa kua arotauhia te punaha tauhohenga, he iti ake te kaha o te whakakaha ake i te 90%, kaore i te kitea te ahua "S" o te pihi whakakaha, he iti te heke, he papatahi te pihi.Ki te iti te nui o te tauira, kaore e taea te whakanui ake, a, ka nui te rahi o te tauira, kaore i te pai te awe whakakaha.Na reira, ko te kowhiringa o nga polymerases DNA me te kaha whakakaha teitei he mea nui ki te angitu o te PCR me te qPCR.

2. Tīpakohia wera-tīmata Taq whākōkī me te kaha pūmua whākōkī

Ko te kaha enzymatic o te Taq enzyme e pa ana ki te kaha o te whakanui.Ko te tikanga, ko te kaha ake o te kaha enzymatic o te wera-tīmatanga Taq whākōkī, te roa te wā tupu taupūtanga o PCR amplification, te ake angamaheni 'S-ahua' ānau, teitei ake te uara tohu fluorescence, me te pai ake mo te rapu PCR multiplex.Ka taea e nga polymerases DNA waitohu me te kaha enzymatic ngoikore te tautoko i nga tauhohenga 2-plex anake.A, no te mahi 3-plex tauhohenga, he iti te ānau amplification, he iti te uara tohu fluorescence, a kahore he ānau amplification angamaheni, na he uaua ki te whakawa nga hua.

3. Tīpakohia he whākōkī Taq tīmata-wera me te tairongo teitei

I te nuinga o te korero, he nui te kaha o te whakakaha o te DNA polymerase me te tairongo nui, engari he rerekee ano.Mena he iti te rahinga o te ira o te tauira hei whakanui ake, e taunaki ana kia whakamatauria te tairongo whakakaha o Taq enzyme.Ko te tikanga rapu tino noa ko te kawe i te waimehatanga 10-tiki, 5-tiki ranei o te wahanga plasmid ira i whaia, ka mahia te kitenga PCR i te waimehatanga o raro, me te kowhiri i te whaariki Taq timata-wera me te aro nui ki te kitenga.

Ka kitea i runga ake nei me whiriwhiri nga kairangahau i runga i o raatau ake whakaritenga whakamatautau me nga tikanga putea.He pai ake te mahi i tetahi whakamatautau whakakaha waimehatanga rōnaki ki te kite i te kaha o te whakakaha me te tairongo o te whaariki Taq timata wera.

He tauira o Foregene's Taq DNA Polymerase:

Foreasy HS Taq DNA Polymerase

Whakaahuatanga

Ko te Foreasy HS Taq DNA Polymerase he DNA polymerase e whakaatuhia ana i roto i te Escherichia coli engineering huakita e te hangarau whakakotahi ira.Ka honoa te enzyme ki te putunga tauhohenga ahurei, na te mea ka tino atete te hua me te hototahi, ka taea te whakamahi tika i te tauira lysate (Foregene Lysis system) hei tauira mo nga tauhohenga kitenga.

Taupānga

PCR tohu me te PCR ine te kitenga o nga tauira kua purea me nga tauira kaore i purea.

Mana Kounga

1.Karekau he mahi karihi o waho i kitea

Tikanga 2.PCR ki te kite i te toenga o te DNA genomic kaore he kaihautu

3.Ka taea e ia te whakanui ake i nga ira kape-kotahi i roto i te Genome tangata

4. Rokiroki i te pāmahana rūma mo te wiki, ka huri nga mahi noobvious

Taipitopito hua: https://www.foreivd.com/foreasy-hs-taq-dna-polymerase-product/