He aha te Direct PCR

Ko te PCR tika te tikanga mo te whakakaha DNA tika mai itoto, kikokiko kararehe,hiku kiore,pūtau,taringa kiore,hepara ika, hua ika,rau tipu,kākanome etahi atu tauira kiko koiora taketake me te kore e mahi i te wehenga DNA me nga mahi purenga.Ka taea e te hangarau PCR tika te whakaiti i te wa whakamatautau, me te utu mo te mahi ira tangata me nga kaupapa nui.He pai ake ano te whiringa ina pa ana ki nga wero o te whakanui i te rahinga iti rawa o te tauira ka taea e te taahi purenga te mate tauira.

He kaha te awangawanga a FOREGENE's PCR Mix, na te kaha o FOREGENE i patented Taq enzyme( patent mana: ZL20161034512.0 (Haina), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)).Ka whakamahia e tana enzyme Taq te hangarau whakakotahi DNA ki te whakakotahi ano i te rohe hanganga here DNA o te pūmua here waikawa karihi archaeal me te Taq DNA polymerase-wera hei hanga i tetahi tauira DNA kua piki ake te hononga.He Taq timata-wera kua pai ake e pupuri ana i nga momo mahi o te DNA polymerase.

For tauira,ithe ngawari ki te taapiri i topūtau,tipu (rau hou, pakiaka, kakano ranei) tauira kararehe ranei ki tepūreirei, me te tāpiri i te ahureiuru rangatira kimotuhakenga mea tuatahi i roto i nga ngongo PCR me te whakahaere i roto i te miihini hiko waiariki.He pai te pai o te kete mo te tātari tauira nui-nui he mea tino nui te penapena wa.

Ko te punaha tauhohenga PCR kei roto ko te UNG enzyme me te dUTP ka tohua i runga i nga whakaritenga whakamatautau, ka taea te karo i te parahanga na nga hua whakakaha PCR.