I whakaputaina e: CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
Mena: 69.504
Hua awhina:Pūtau Tapeke RNA Wehewehenga Kete
Hononga Taketake:https://www.nature.com/articles/s41586-022-05271-2
Ko nga mate ki te filoviruses penei i te Ebola virus (EBOV) me te Marburg huaketo ka puta he tohu haumanu kino, tae atu ki te kirikaa haemorrhagic me te ngoikoretanga o nga whekau maha.4.I konei ka whakatauhia e matou te hanganga o te Ebola huaketo L pūmua i roto i te matatini me te tetrameric VP35 ma te whakamahi i te karuiti cryo-electron (state 1).I kitea e te tātaritanga hanganga ko te huaketo Ebola L kei a ia tetahi huānga whakauru filovirus-motuhake he mea tino nui mo te whakahiato RNA, a ko te VP35 e mahi tahi ana me te rohe N-terminal o L e toru nga protomer o te VP35 tetramer.
I tenei pepa, i tangohia te tapeke RNA ma te whakamahi i te ForegenePūtau Tapeke RNA Wehewehenga Ketea ko te kukū o te RNA i inehia e Nanodrop i te 260 nm, ka mahi i te RT / qPCR rapunga.
■ Ko te tukanga katoa e whakahaerehia ana i te pāmahana rūma (15-25 ℃), kaore he pati hukapapa me te iti o te wera o te centrifugation.
■ Ko te katoa o te kete he RNase-Free, kaua e manukanuka ki te whakahekenga o te RNA.
■ Ko te Tiwae Horoi A-DNA e herea ana i te DNA, kia taea ai e te kete te tango i te parahanga DNA ira me te kore e taapiri atu i te DNase.
■ Hua RNA teitei: Ko te Tiwae RNA-anake me te tauira ahurei ka taea te pure i te RNA.
■ Tere tere: ngawari ki te whakahaere, ka taea te whakaoti i roto i te 11 meneti.
■ Haumaru: Kaore e hiahiatia he reagent pararopi.
■ Te kounga teitei: Ko te RNA kua purea he tino parakore, kaore he pūmua me etahi atu poke, a ka taea e ia te whakatutuki i nga momo whakamatautau ka whai ake.
Wā tuku: Noema-04-2022
