Kounga Teitei mo Haina PCR Tauira Whakaritenga Tangohanga Tangohanga Waikawa Nucleic Aunoa
Ko te whiwhi whakatutukitanga a nga kaihoko ko te kaupapa a ta maatau kamupene kaore he mutunga.Ka mahia e matou he kaupapa nui ki te whiwhi otinga hou me te kounga teitei, te whakatau i o whakaritenga motuhake me te whakarato ki a koe i mua i te hoko, i runga i te hoko me nga kaiwhakarato muri-hoko mo te Kounga teitei mo Haina PCR Tauira Whakaritenga Tango Whakamaori Waikawa Nucleic Aunoa, Ko te kounga teitei, nga ratonga tika me te utu hoko kaha, ka riro katoa i a matou te rongonui nui i te mara xxx ahakoa te whakataetae o te ao.
Ko te whiwhi whakatutukitanga a nga kaihoko ko te kaupapa a ta maatau kamupene kaore he mutunga.Ka mahia e matou he kaupapa tino pai ki te whiwhi otinga hou me te tino kounga, te whakatau i o whakaritenga motuhake me te whakarato ki a koe i mua i te hoko, i runga i te hoko me nga kaiwhakarato muri-hoko moHaina Infrared Thermometer, Taputapu Ora-Putaiao, kua 8 tau o matou wheako ki te whakaputa me te 5 tau te wheako ki te hokohoko me nga kaihoko puta noa i te ao.ko o taatau kaihoko te nuinga o te tohatoha i Amerika Te Tai Tokerau, Awherika me te Rawhiti o Uropi.ka taea e matou te tuku hua kounga teitei me te utu tino whakataetae.
Whakatakotoranga
48 Rxns, 96 Rxns
| Kao. | Waehanga | Te nui | Nga waahanga matua | |||
| 48 rxn | 96 rxn | |||||
| 1 | He kaihoko tuku waikawa nucleic | 1.4mL/ngongo | 2 ngongo | 5.3 mL/pounamu | 1 pounamu | Surfactant |
| 2 | RNA tiaki | 27 μL/ngongo | 1 ngongo | 53 μL/ngongo | 1 ngongo | te aukati RNase |
| 3 | otinga tauhohenga SARS-CoV-2 | 800 μL / ngongo | 1 ngongo | 1600 μL/ngongo | 1 ngongo | Primer, probe, parepare tauhohenga, dNTP |
| 4 | Te ranunga whākōkī SARS-CoV-2 | 80 μL/ngongo | 1 ngongo | 160 μL/ngongo | 1 ngongo | Hot start Taq enzyme, M-MLV enzyme |
| 5 | SARS-CoV-2 mana pai | 100 μL/ngongo | 1 ngongo | 100 μL/ngongo | 1 ngongo | Ko te plasmid recombinant kei roto ko te mahanga whaainga, RNA |
| 6 | SARS-CoV-2 mana kino | 1200 μL/ngongo | 1 ngongo | 1200 μL/ngongo | 1 ngongo | TE parepare |
Nga waahanga me nga painga
Tere me te ngawari.
A ape i te mate waikawa karihi.
He iti te rohe kitenga me te tairongo teitei.
He iti te hiahia o nga taputapu me te whakamahi tino ngawari.
Ka tae te tairongo ki te taumata iti rawa ki te 500 kape/mL).
| GCE/mL | Uara Ct | |
| N ira | ORF1ab ira | |
| 2.00E+08 | 16.43 | 15.16 |
| 2.00E+07 | 19.48 | 18.58 |
| 2.00E+06 | 22.71 | 21.86 |
| 2.00E+05 | 25.95 | 25.33 |
| 2.00E+04 | 29.30 | 28.49 |
| 2.00E+03 | 32.37 | 31.37 |
| 2.00E+02 | 35.32 | 34.47 |
| 2.00E+01 | 38.29 | 38.27 |
| 2.00E+00 | N/A | N/A |
| 2.00E-01 | N/A | N/A |
| NC | N/A | N/A |
Anau whakakaha raina
Me pehea te whakamahi
1. taputapu e tika ana
2. Te tikanga rapu
Tukatuka tauira: Tauira kaukau naopharyngeal or oropharyngeal swab (1 anake te mahi)(Tatari mo te 10 meneti)
Whakanui PCR(<=55min)
Rerengamahi:
Rokiroki
hiritia mai i te marama ka rongoa ki te -20±5 ℃;
ora papa:1 tau
Ko te whiwhi whakatutukitanga a nga kaihoko ko te kaupapa a ta maatau kamupene kaore he mutunga.Ka mahia e matou he kaupapa nui ki te whiwhi otinga hou me te kounga teitei, te whakatau i o whakaritenga motuhake me te whakarato ki a koe i mua i te hoko, i runga i te hoko me nga kaiwhakarato muri-hoko mo te Kounga teitei mo Haina PCR Tauira Whakaritenga Tango Whakamaori Waikawa Nucleic Aunoa, Ko te kounga teitei, nga ratonga tika me te utu hoko kaha, ka riro katoa i a matou te rongonui nui i te mara xxx ahakoa te whakataetae o te ao.
Kounga teitei moHaina Infrared Thermometer, Taputapu Ora-Putaiao, kua 8 tau o matou wheako ki te whakaputa me te 5 tau te wheako ki te hokohoko me nga kaihoko puta noa i te ao.ko o taatau kaihoko te nuinga o te tohatoha i Amerika Te Tai Tokerau, Awherika me te Rawhiti o Uropi.ka taea e matou te tuku hua kounga teitei me te utu tino whakataetae.
