Ko o tatou maataapono te kounga teitei me te tino pai, ka awhina i a tatou ki te tuunga rangatira.Ko te piri ki to kaupapa "kounga tuatahi, tino nui o te kiritaki" mo te Kaituku Ipurangi Viru Tangohanga Tikanga Piira Aukume Tikanga Viral DNA / Rna Nucleic Acid Tangohanga Reagent Kits mo te Tirohanga PCR Waa me te CE, I te wa e whakamahi ana i te whakapai ake o te hapori me te ohanga, ka mau ta taatau hinonga i te kaupapa "Arotahi ki te whakawhirinaki, te kounga teitei te tuatahi", me te mea he roa taatau ki te whakahaere i ia kaihoko.
Ko o tatou maataapono te kounga teitei me te tino pai, ka awhina i a tatou ki te tuunga rangatira.Te u ki to kaupapa "kounga tuatahi, tino rangatira" moHaina Toto DNA me te Kete Tangohanga, Kei te tohe to taatau kamupene ki te kaupapa o te "whakanuia te ratonga mo te paerewa, te taurangi kounga mo te waitohu, te mahi pakihi i runga i te whakapono pai, ki te tuku ratonga tohu, tere, tika me te wa tika mo koe".Ka mihi matou ki nga kaihoko tawhito me nga kaihoko hou ki te whakawhitiwhiti korero ki a matou.Ka mahi matou ki a koe me te pono!

Whakatakotoranga

200 ×20μl rxns, 2000 ×20μl rxns 2× SuperEasy^TMWhakaranu-EDTA: Kei roto te D-Taq DNA Polymerase, dNTPs, MgCl2, tauhohenga parepare, PCR tauhohenga whakarei, optimizer me te stabilizer, me era atu.₂O ki te 2× SuperEasy^TMWhakaranu-EDTA hei whakamahi i te tauhohenga PCR.

6× DNA Loading Buffer: Karekau he SDS i roto i te Buffer Loading.E taunaki ana ki te whakamahi i te 6× DNA Loading Buffer i whakauruhia ki te kete i te wa e mahi ana i te electrophoresis gel agarose kia whiwhi ai i nga hua electrophoresis pai.Kaua e whakamahi i te Utatanga Buffer kei roto SDS, ki te kore ka nui te hiku o te rama kanapa i roto i te huarahi i te wa o te electrophoresis, ka pa ki nga hua whakamatautau.

Nga waahanga kete

Nga waahanga me nga painga

-Kare he hiahia mo te purenga DNA e pau ana te wa me te utu nui, kaore he maimoatanga o mua, me te whakamahi tika i te toto katoa hei tauira PCR.-He kaha te kaha whakakaha o te punaha, a ka taea e ia te kite i nga wahanga DNA i roto i te toto.-2× SuperEasy^TMWhakaranu-EDTA Te manawanui o te toto: tae atu ki te 45% i roto i te toto tangata me te 20% i te toto kiore.-Ko te tauira kua kapi katoa, na reira kaore he take e awangawanga mo te pokenga tauira me nga hua PCR pai.

Te tono kete

I whakatapua ki te tautuhi PCR tika mo te EDTA anticoagulated toto katoa.(Tae atu: toto tangata, toto kiore, toto heihei, toto manu, toto kau, toto kuri, etc.)

Rerengamahi

Rokiroki me te ora o te Papa

Me penapena tenei kete ki te -20°C.2× SuperEasy^TMWhakaranu-EDTA, ki te whakamahia auau, ka taea hoki te penapena ki te 4 ℃ mo te rokiroki mo te wa poto (whakamahia i roto i nga ra 10).6× DNA Loading Buffer，Ka taea te penapena ki te 4°C, -20°C ranei mo te wa roa. Ko o tatou maataapono o te kounga teitei me te tino pai te tuunga nama, ka awhina i a tatou ki te tuunga o runga.Ko te piri ki to kaupapa "kounga tuatahi, tino nui o te kiritaki" mo te Kaituku Ipurangi Viru Tangohanga Tikanga Piira Aukume Tikanga Viral DNA / Rna Nucleic Acid Tangohanga Reagent Kits mo te Tirohanga PCR Waa me te CE, I te wa e whakamahi ana i te whakapai ake o te hapori me te ohanga, ka mau ta taatau hinonga i te kaupapa "Arotahi ki te whakawhirinaki, te kounga teitei te tuatahi", me te mea he roa taatau ki te whakahaere i ia kaihoko.
Kaituku IpurangiHaina Toto DNA me te Kete Tangohanga, Kei te tohe to taatau kamupene ki te kaupapa o te "whakanuia te ratonga mo te paerewa, te taurangi kounga mo te waitohu, te mahi pakihi i runga i te whakapono pai, ki te tuku ratonga tohu, tere, tika me te wa tika mo koe".Ka mihi matou ki nga kaihoko tawhito me nga kaihoko hou ki te whakawhitiwhiti korero ki a matou.Ka mahi matou ki a koe me te pono!