PCR Heroᵀᴹ
Whakaahuatanga kete:
◮Te pono teitei ake: 6he taima ki tera o Taq enzyme noa;
◮Tere ake atere whakaemi:ko te tere whakakaha he 5-10sec/kb, he 6-12 nga wa o te Taq enzyme noa;
◮He nui ake te urutau tauira: ka taea e ia te whakanui ake i nga momo tauira DNA matatini me nga ihirangi GC teitei me te uaua ki te whakanui;
◮Teitei akeTe kaha whakakaha: he iti ake te maha o nga huringa whakakaha ake i tera o Taq enzyme noa;
◮Staratara Te manawanui o te taiao: ka whakanohoia ki te 37°C mo te wiki, kia nui ake te pupuri90%mahi;
◮He 5'→3' te mahi polymerase DNA me te 5'→3' mahi exonuclease, kaore he mahi exonuclease 3'→5'.
Whakaahuatanga kete
Kei roto i tenei hua te reanga hou ahurei a Foregene o Taq——Foregene Taq Hero, nga dNTP kua arotau, MgCl2, parepare tauhohenga, whakarei tauhohenga PCR, arotau me te whakapumau.Ko te 2 × PCR HeroTMHe teitei ake te manawanui o te punaha whakaranu ki nga aukati PCR i te punaha PCR Mix noa, a ka ngawari ki te whakatutuki i te whakakaha PCR o nga momo tauira uaua.Ko te punaha tauhohenga ahurei me te kaha nui o Taq Hero ka nui ake te kaha o te whakakaha, te motuhake me te tairongo o te tauhohenga PCR.
Whakatakotoranga
Raupapa kete
PH-01013：2× PCR HeroTMWhakaranuhia te 1.7ml ×3
PH-01014：2× PCR HeroTMWhakaranuhia te 1.7ml ×6
PH-01015：2× PCR HeroTMWhakaranuhia te 1.7ml ×24
Nga waahanga kete
2× PCR HeroTMWhakaranu ko: 0.1U Taq Hero/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8),100mM KCl, 5mM MgCl2,etahi atu whakapumau me te whakarei.
Nga waahanga me nga painga
-Te pono teitei ake:6he taima ki tera o Taq enzyme noa;
- Tere ake atere whakaemi:ko te tere whakakaha he 5-10sec/kb, he 6-12 nga wa o te Taq enzyme noa;
-He nui ake te urutau tauira: ka taea e ia te whakanui ake i nga momo tauira DNA matatini me nga ihirangi GC teitei me te uaua ki te whakanui;
- Teitei akeTe kaha whakakaha: he iti ake te maha o nga huringa whakakaha ake i tera o Taq enzyme noa;
-Staratara Te manawanui o te taiao: ka whakanohoia ki te 37°C mo te wiki, kia nui ake te pupuri90%mahi;
-He 5'→3' te mahi DNA polymerase me te 5'→3' mahi exonuclease, kaore he mahi exonuclease 3'→5'.
Te tono kete
- PCR te whakanui ake i nga wahanga DNA
- Tapanga DNA
- Te raupapa DNA
- PCR me te hiku
Te rokiroki me te oranga o te papa
Ka taea te penapena mo te wa roa ki te -20°C;mo te whakamahi auau, ka taea te rongoa ki te 4°C mo te wa poto.
