Utu korero mo te Tapeke Rna Whakamuri Whakamuri Kete Whakamaori Mirna First Strand Cdna Synthesis Kit Tailing
E whakapono ana matou: Ko te auahatanga to tatou wairua me to tatou wairua.Ko te kounga teitei ko to tatou oranga.Ko te hiahia o te kaihoko ko to tatou Atua mo te utu utu mo te Tapeke Rna Whakamuri Whakamuri Kete Whakamaori Mirna First Strand Cdna Synthesis Kit Tailing, Nau mai ki te toro mai ki a matou i nga wa katoa mo te marena whakahaere.
E whakapono ana matou: Ko te auahatanga to tatou wairua me to tatou wairua.Ko te kounga teitei ko to tatou oranga.Ko te hiahia o te kaihoko ko to tatou AtuaHaina Tapeke Rna Whakamuri Whakamuri me te Cdna Synthesis Tailing Kit, He matatau te whakaputa me nga taputapu tukatuka me nga kaimahi mohio ki te whakarite i nga taonga me te kounga teitei.Kua kitea e matou he ratonga pai i mua i te hoko, hoko, muri-hoko hei whakarite i nga kaihoko ka kaha ki te whakarite ota.Tae noa ki tenei wa kei te tere haere tonu a maatau otinga me te tino rongonui ki Amerika ki te Tonga, ki te Rawhiti o Ahia, ki te Rawhiti Waenganui, ki Awherika, aha atu.
Whakatakotoranga
5ml, 10ml, 40ml
PCR Hero (Me te Waikano) kete tangohanga kei roto ko te reanga hou ahurei a Foregene o Taq—Foregene Taq Hero, nga dNTP kua arotauhia, MgCl2, te parepare tauhohenga, te whakahiato tauhohenga PCR, te whakarorohiko me te whakapumau.Ko te punaha 2× PCR HeroTM Mix (waikano) he nui ake te manawanui ki te aukati PCR i te punaha PCR Mix noa, a ka ngawari ki te whakatutuki i te whakakaha PCR o nga momo tauira uaua.Ko te punaha tauhohenga ahurei me te kaha nui o Taq Hero ka nui ake te kaha o te whakakaha, te motuhake me te tairongo o te tauhohenga PCR.
Nga waahanga kete
0.1U Taq Hero/μl |
400μM dNTPs |
20mM Tris-HCl(pH8.8) |
100mM KCl |
5mM MgCl2 |
Ētahi atu pūmau me te whakarei ake |
Puawai Uta DNA |
Nga waahanga me nga painga
■ Te pono teitei ake: 6 nga wa i to te Taq enzyme noa;
■ Ko te tere whakakaha ake : ko te 5-10sec/kb te tere whakakaha, ko te 6-12 nga wa o te Taq enzyme noa;
■ He pai ake te urutau tauira: Ka taea e ia te whakanui ake i nga momo tauira DNA matatini me te teiteiihirangi GC;
■ He pai ake te kaha o te whakakaha: he iti ake nga huringa whakakaha i to Taq enzyme noa;
■ He kaha ake te manawanui o te taiao: ka whakanohohia ki te 37°C mo te wiki, me te pupuri i nga mahi neke atu i te 90%;
■ He 5′ →3′ DNA polymerase mahi me te 5′→3′ mahi exonuclease, kahore he 3′ →5′ mahi exonuclease.
■ Ko te kete i mua i konatunatua ki te Electrophoresis Loading Buffer, ka oti te PCR, ka taea te utaina tika te tauira mo te electrophoresis;he ngawari, he ngawari hoki te mahi.
Te tono kete
Ko te whakakaha PCR o nga kongakonga DNA, te tapanga DNA, te raupapa DNA, te PCR me te hiku A
Rokiroki me te ora o te Papa
Ka taea te penapena mo te wa roa ki te -20°C;mo te whakamahi auau, ka taea te rongoa ki te 4°C mo te wa poto. E whakapono ana matou: Ko te auahatanga to tatou wairua me to tatou wairua.Ko te kounga teitei ko to tatou oranga.Ko te hiahia o te kaihoko ko to tatou Atua mo te utu utu mo te Tapeke Rna Whakamuri Whakamuri Kete Whakamaori Mirna First Strand Cdna Synthesis Kit Tailing, Nau mai ki te toro mai ki a matou i nga wa katoa mo te marena whakahaere.
Ko te utu utu moHaina Tapeke Rna Whakamuri Whakamuri me te Cdna Synthesis Tailing Kit, He matatau te whakaputa me nga taputapu tukatuka me nga kaimahi mohio ki te whakarite i nga taonga me te kounga teitei.Kua kitea e matou he ratonga pai i mua i te hoko, hoko, muri-hoko hei whakarite i nga kaihoko ka kaha ki te whakarite ota.Tae noa ki tenei wa kei te tere haere tonu a maatau otinga me te tino rongonui ki Amerika ki te Tonga, ki te Rawhiti o Ahia, ki te Rawhiti Waenganui, ki Awherika, aha atu.