Ko te RT-PCR he hangarau e whakakotahi ana i te whakamuri whakamuri RT o te RNA me te polymerase chain amplification (PCR) o te cDNA.Ko te hangarau RT-PCR he tairongo me te whai kiko, ka taea te whakamahi ki te kite i te taumata o te whakaaturanga ira i roto i nga pūtau/kopa, te ihirangi o nga wheori RNA i roto i nga pūtau, me te whakakoi tika i nga raupapa cDNA o nga ira motuhake.
TE PAINGA MAHI:
✱ Ko te kete RT a Foregene he tino pumau i te 50-65 ℃ te pāmahana tauhohenga (ka taea e te 65°C te whakatuwhera i te taumata tuarua o te RNA kia pai ai te haere o te tauhohenga whakawhiti whakamuri)
✱ He kaha te manawanui, ka taea te aro ki etahi waihano me te tote guanidine
iti-Rangatū | Ingoa Hua | Whakatakotoranga | Nama Putumōhio | Nga Tikanga Rokiroki |
PCR Mix Series | 5ml | PH-01013 | -20 ℃ | |
40ml | PH-01015 | |||
5ml | PH-01013-B | -20 ℃ | ||
40ml | PH-01015-B | |||
1ml | DP-20031 | -20 ℃ | ||
3×1.7ml | DP-20032 | |||
3×1.7ml | DP-20032-B | -20 ℃ | ||
50ml | DP-20034-B | |||
2×Rapid PCR tauhohenga premix system 2× PCR Mix | 1ml | DP-20041 | -20 ℃ | |
3×1.7ml | DP-20042 | |||
500 ×20μl rxns | QP-01012 | -20 ℃ | ||
2000 ×20μl rxns | QP-01014 | |||
500 ×20μl rxns | QP-01022 | -20 ℃ | ||
2000 ×20μl rxns | QP-01024 | |||
Raupapa RT | 25× 20μl Tauhohenga | RT-01011 | -20 ℃ | |
100 × 20μl Tauhohenga | RT-01012 | |||
(Master Premix mo te whenu tuatahi cDNA synthesis mo PCR Wā Tuturu) | 200 × 10μl Tauhohenga | RT-01022 | -20 ℃ | |
800 × 10μl Tauhohenga | RT-01023 | |||
Master Premix mo te whenu tuatahi cDNA synthesis mo te PCR Wā Tuturu me te gDNase | 100 × 20μl Tauhohenga | RT-01032 | -20 ℃ | |
Lnc-RT HeroTM I(Me te gDNase)(Super Premix mo te whenu tuatahi cDNA synthesis mai i lncRNA) | 25 × 20μl Tauhohenga | RTL-09098 | -20 ℃ | |
50 × 20μl Tauhohenga | RTL-09099 | |||
RT-PCR Series | 100 rxn | RT-02011 | -20 ℃ | |
500 rxn | RT-02012 | |||
100 rxn | RT-02021 | -20 ℃ | ||
500 rxn | RT-02022 | |||
RT-qPCR Series | 100 rxn | RT-02111 | -20 ℃ | |
500 rxn | RT-02112 | |||
100 rxn | RT-02131 | -20 ℃ | ||
500 rxn | RT-02132 | |||
Tohu DNA | 50 Whakaritenga | DL-20011 | 4℃ | |
200 Whakaritenga | DL-20012 | |||
50 Whakaritenga | DL-30011 | 4℃ | ||
200 Whakaritenga | DL-30012 |