PCR Heroᵀᴹ (Me te Waikano)
Whakaahuatanga kete:
◮Te pono teitei ake: e 6 nga wa i to Taq enzyme noa;
◮Tere whakakaha tere
◮He nui ake te urutau tauira
◮He pai ake te whakakaha ake
◮He kaha ake te manawanui o te taiao: ka whakanohoia ki te 37 ° C mo te wiki, me te pupuri i nga mahi neke atu i te 90%;
◮He 5'→3' te mahi polymerase DNA me te 5'→3' mahi exonuclease, kaore he mahi exonuclease 3'→5'.
Whakatakotoranga
5ml, 10ml, 40ml
PCR Hero (Me te Waikano) kete tangohanga kei roto ko te reanga hou ahurei a Foregene o Taq—Foregene Taq Hero, nga dNTP kua arotauhia, MgCl2, te parepare tauhohenga, te whakahiato tauhohenga PCR, te whakarorohiko me te whakapumau.Ko te punaha 2× PCR HeroTM Mix (waikano) he nui ake te manawanui ki te aukati PCR i te punaha PCR Mix noa, a ka ngawari ki te whakatutuki i te whakakaha PCR o nga momo tauira uaua.Ko te punaha tauhohenga ahurei me te kaha nui o Taq Hero ka nui ake te kaha o te whakakaha, te motuhake me te tairongo o te tauhohenga PCR.
Nga waahanga kete
| 0.1U Taq Hero/μl |
| 400μM dNTPs |
| 20mM Tris-HCl(pH8.8) |
| 100mM KCl |
| 5mM MgCl2 |
| Ētahi atu pūmau me te whakarei ake |
| Puawai Uta DNA |
Nga waahanga me nga painga
■Te pono teitei ake: e 6 nga wa i to Taq enzyme noa;
■ Ko te tere whakakaha ake : ko te 5-10sec/kb te tere whakakaha, ko te 6-12 nga wa o te Taq enzyme noa;
■ He pai ake te urutau tauira: Ka taea e ia te whakanui ake i nga momo tauira DNA matatini me te teiteiihirangi GC;
■ He pai ake te kaha o te whakakaha: he iti ake nga huringa whakakaha i to Taq enzyme noa;
■ He kaha ake te manawanui o te taiao: whakanohoia ki te 37°C mo te wiki, neke atu i te 90% mahi;
■ He 5'→3'He mahi polymerase DNA me te 5'→3'mahi exonuclease, kaore he 3'→5'mahi exonuclease.
■ Ko te kete i mua i konatunatua ki te Electrophoresis Loading Buffer, ka oti te PCR, ka taea te utaina tika te tauira mo te electrophoresis;he ngawari, he ngawari hoki te mahi.
Te tono kete
Ko te whakakaha PCR o nga kongakonga DNA, te tapanga DNA, te raupapa DNA, te PCR me te hiku A
Rokiroki me te ora o te Papa
Ka taea te penapena mo te wa roa ki te -20°C;mo te whakamahi auau, ka taea te penapena ki te 4°C mo te wa poto.
