Ko te Kete Tirohanga Waikawa Nucleic Variant SARS-CoV-2 (I) (Multiplex PCR Fluorescent Probe Tikanga)-mo te kite i nga mutant mai Brazil, Awherika ki te Tonga, me UK
Whakaahuatanga kete:
◮ PCR Tika:Kaore e hiahiatia he kete tangohanga waikawa karihi me te Pūnaha, kua oti te 96 tauira i roto i te 1 haora neke atu ranei.
◮ He iti te hiahia o nga taputapu me te tino ngawari te whakamahi:Me hiahia noa te punaha PCR Wā-Tuturu.
◮ LoD me te tairongo teitei:Ka tae ki te taumata iti rawa te tairongo ki te 500 kape/ml.
20+ tau R&D Wheako Hangahanga 25+ teitei ake te take paanga
1000+ rerekē taiwhanga tauhohenga 100+ whare wānanga hoa
400+ kaitoha 2000+ tohutoro SCI o nga reagents taiwhanga
Whakatakotoranga
48 Rxns/kit, 96 Rxns/kit
| Kao. | Waehanga | Te nui | Te whakauru matua | |||
| 48 rxn | 96 rxn | |||||
| 1 | He kaihoko tuku waikawa nucleic | 5.3 mL/ pounamu | 1 pounamu | 5.3 mL/pounamu | 2 pounamu | Surfactant |
| 2 | RNA tiaki | 53 μL/ngongo | 1 ngongo | 106 μL/ngongo | 1 ngongo | te aukati RNase |
| 3 | SARS-CoV-2 otinga tauhohenga A | 800 μL / ngongo | 1 ngongo | 1600 μL/ngongo | 1 ngongo | Primer, probe, parepare tauhohenga, dNTP |
| 4 | SARS-CoV-2 otinga tauhohenga B | 800 μL / ngongo | 1 ngongo | 1600 μL/ngongo | 1 ngongo | Primer, probe, parepare tauhohenga, dNTP |
| 5 | Te ranunga whākōkī SARS-CoV-2 | 160 μL/ngongo | 1 ngongo | 320 μL/ngongo | 1 ngongo | Hot start Taq enzyme, M-MLV enzyme |
| 6 | SARS-CoV-2 whakahaere pai | 100 μL/ngongo | 1 ngongo | 100 μL/ngongo | 1 ngongo | Ko te plasmid recombinant kei roto ko te mahanga whaainga, RNA |
| 7 | SARS-CoV-2 mana kino | 1200 μL/ngongo | 1 ngongo | 1200 μL/ngongo | 1 ngongo | TE parepare |
Tuhipoka: Ko nga waahanga mai i nga kete rereke kaore e taea te whakamahi rereke.
Nga waahanga me nga painga:
Tere me te ngawari: whakaotihia te whakamatautau mo te 1 haora
A ape i te mate waikawa karihi: kaore he take mo te purenga waikawa karihi
He iti te rohe rapunga me te tairongo teitei, ina koa he pai mo te kitenga tere nui
He iti te hiahia o nga taputapu me te whakamahi tino ngawari
Ka tae te tairongo ki te taumata iti rawa ki te 500 kape/mL).
Me pehea te whakamahi:
1.Whakaahua taputapu
ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S
2. Te mahi kimi
Tukatuka tauira: Tauira kaukau naopharyngeal or oropharyngeal swab (1 taahiraa anake te mahi)(Tatari mo te 10 meneti)
Whakanui PCR(<=55min)
Rerengamahi:
Rokiroki:
I hirihia mai i te marama me te rongoa ki te -20±5 ℃;
Rokiroki i te 2-8 ℃ i muri i te whakatuwheratanga, whakamahia i roto i nga ra 7;karohia te tio-rewa tonu (<5 nga wa)
ora papa:1 tau
