Serum(Plasma) miRNA Kete Wehewehenga
Whakaahuatanga kete:
◆Ko te kete huinga katoa RNase-Kore, kaua e awangawanga mo te whakahekenga o te RNA.
◆Ko te Tiwae Whakapai-Ana ka herea ki te DNA, kia taea ai e te kete te tango i te poke DNA ira me te kore e taapiri atu i te DNase.
◆Hua RNA teitei: Ko te Tiwae RNA-anake me te tauira ahurei ka taea te pure i te RNA.
◆Tere tere: He ngawari te mahi ka taea te whakaoti i roto i te 30 meneti.
◆Haumaru: Karekau he tangohanga reagent waro e hiahiatia ana.
◆Kounga teitei: Ko nga kongakonga RNA kua tangohia he tino parakore, he kore he pūmua me etahi atu poke, a ka taea e ia te whakatutuki i nga momo tono whakamatautau o raro.
Whakaahuatanga
Ka whakamahia e tenei kete te pou hurihuri me te tauira i hangaia e ta maatau kamupene, ka taea te tango i nga kongakonga iti o te miRNA iti iho i te 100nt mai i te serum me te plasma.Ko te kete e whakarato ana i te Tiwae Whakapai DNA, ka taea te wehe ngawari i te supernatant me te kiko lysate me te adsorb me te tango i te DNA i roto i te tauira, he mea ngawari me te wa-whakaora;Ka taea e te Tiwae RNA-anake te here i te RNA, me te tauira ahurei, ka taea te whakamahi i te wa ano.Tukatia te maha o nga tauira.
Ko te punaha katoa he RNase-Free, kia kore ai te miRNA kua purea e whakaheke;Ko te Buffer miBRW1 me te Buffer miBRW2 te punaha horoi parapara ka whiwhi te RNA me te kore he pūmua, DNA, katote, me te parahanga pūhui waro.
Whakatakotoranga
5T, 50T, 100T, 200T
Nga waahanga kete
| Serum/Plasma miRNA Kete Wehewehenga | ||||
| Kei roto kete | RE-0111T | RE-01111 | RE-01112 | RE-01114 |
| 5 T | 50 T | 100T | 200T | |
| Pupuri miBRL1* | 2ml | 20ml | 40ml | 80ml |
| Pupuri miBRL2* | 1.2ml | 12ml | 24ml | 48ml |
| Pupuri miBRW1 | 2ml | 20ml | 40ml | 80ml |
| Pupuri miBRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| RNA-anake Tīwae | 5 mongamonga | 50mongamonga | 100mongamonga | 200mongamonga |
| Tiwae Whakapai DNA | 5 mongamonga | 50mongamonga | 100mongamonga | 200mongamonga |
| IFU | 1 wahi | 1 wahi | 1 wahi | 1 wahi |
Nga waahanga me nga painga
◆Ko te kete huinga katoa RNase-Kore, kaua e awangawanga mo te whakahekenga o te RNA.
◆Ko te Tiwae Whakapai-Ana ka herea ki te DNA, kia taea ai e te kete te tango i te poke DNA ira me te kore e taapiri atu i te DNase.
◆Hua RNA teitei: Ko te Tiwae RNA-anake me te tauira ahurei ka taea te pure i te RNA.
◆Tere tere: He ngawari te mahi ka taea te whakaoti i roto i te 30 meneti.
◆Haumaru: Karekau he tangohanga reagent waro e hiahiatia ana.
◆Kounga teitei: Ko nga kongakonga RNA kua tangohia he tino parakore, he kore he pūmua me etahi atu poke, a ka taea e ia te whakatutuki i nga momo tono whakamatautau o raro.
Tawhā kete
Taupānga kete
He pai mo te tangohanga me te purenga o te miRNA mai i te serum kararehe me te tangata me te plasma.
Te tono miRNA
Ko te miRNA kua purea e Serum/Plasma miRNA Isolation Kit ka taea te whakamahi i roto i nga momo whakamatautau irapoi whakararo, penei: RT-PCR, Real Time RT-PCR, Northern Blot, tātari maramara, aha atu.
Te rere mahi
Nga tikanga rokiroki
Ka taea te penapena i tenei kete mo te 12 marama i raro i nga tikanga maroke i te pāmahana rūma(15-25°C).If me penapena mo te wa roa,tēnā koaka rongoa ki te 2-8°C.
