Te Kete Whakamawehenga ADN Whakatipu Kawa Kawa Tipu Tipu DNA Purification Kits Reagents Protocol
Whakatakotoranga
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Ka whakamahia e tenei kete he pou DNA-anake ka taea te here i te DNA, Foregene protease me tetahi punaha parepare ahurei, e tino whakangwari ai te purenga o te DNA genomic tipu.Ka taea te whiwhi i te DNA genomic-kounga teitei i roto i te 30 meneti, ka karo i te paheketanga o te DNA genomic.
Ko te DNA-anake kiri kiri kirikiri e whakamahia ana i roto i te pou hurihuri ko te taonga hou ahurei a Foregene, ka taea te here pai me te tino here ki te DNA, me te whakanui i te tangohanga o te RNA, nga pūmua poke, nga katote, polysaccharides, polyphenols me etahi atu puhui waro.
Nga waahanga hua
| Pūrei PL1, Pūrei PL2 |
| Kaipupuri PW, Kaipara WB, Kaipara EB |
| Foregene Protease |
| DNA-anake Tīwae |
| Tohutohu |
Nga waahanga me nga painga
■ Karekau he poke RNase: Ko te Tiwae DNA-anake e whakaratohia ana e te kete ka taea te tango i te RNA mai i te DNA genomic me te kore RNase taapiri i te wa o te whakamatautau, kia kore ai e poke te taiwhanga e te RNase o waho.
■ Tere tere: He kaha ake te mahi a Foregene Protease i nga momo porotease rite, ka tere ake te keri i nga tauira kiko.
■ Maamaa: ka taea te mahi tangohanga DNA genomic i roto i te 30 meneti.
■ Watea: Ka mahia te centrifugation i te pāmahana rūma, kaore e hiahiatia te 4 ℃ te whakamaarama i te wera iti, te waihano ranei o te DNA.
■ Haumaru: karekau he reagent waro e hiahiatia.
■ Te kounga teitei: Ko te DNA genomic kua purea he maha nga waahanga, kaore he RNA, kahore he RNase, he iti rawa te ihirangi katote, ka taea te whakatutuki i nga whakaritenga o nga momo whakamatautau.
Te tono kete
He pai mo te tangohanga me te purenga o te DNA genomic mai i nga kopa tipu hou, tio ranei.
Te rere mahi
Hoahoa
Rokiroki me te ora o te Papa
Ka taea te penapena i te kete mo te 12 marama ki te pāmahana rūma (15–25 ℃) me te 2–8 ℃ mo te wa roa.
Ko te otinga o Foregene Protease Plus he tauira ahurei, e kaha ana ina rongoa ki te pāmahana rūma mo te wa roa (3 marama);ka pai ake tana mahi me te pumau ka penapena ki4 ℃, no reira ka tūtohu kia penapena ki te 4 ℃, me mahara kia kaua e penapena i te -20 ℃.
He Aratohu Taatari Raruraru
The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.
He iti, he kore DNA ranei
He maha nga mea e pa ana ki te whakaputanga o te DNA genomic, tae atu ki te puna o te tauira, te tau o te tauira, nga tikanga rokiroki o te tauira, me te mahi.
Ko te DNA genomic kaore i taea te tiki i te wa tangohanga
1. Ko nga tauira o te kiko kei te hee te penapena, te penapena ranei mo te roa rawa, ka paheke te DNA genomic.
Taunakitanga: Whakaorangia nga tauira kiko ki roto i te hauota wai, -20 ranei°C;ngana ki te whakamahi i nga tauira hou i kohia mo te tangohanga DNA ira.
2. He iti rawa te rahinga tauira ka kore e tangohia te DNA genomic e rite ana.
Huatau: Mo nga tauira kiko kua roa e rongoa ana, kua tino paheke te DNA ira tangata, ka taea te whakanui i te nui o nga tauira kiko kia taea ai te tango i te DNA ira tangata.Ko te nui o te tauira ka taea te whakatau i runga i nga hiahia DNA, engari ko te tauira hou kaua e neke atu i te 100mg, me te tauira maroke kaua e neke atu i te 30mg.
3. Karekau te tauira e kuia ki te hauota wai, ka waiho ranei mo te roa rawa i muri i te hauota wai.
Huatau: I te wa o te tangohanga DNA, me tino oneone te tauira ki te hauota wai hei pakaru i te pakitara o te pūtau;i muri i te huri, whakawhitia te tauira paura ki te PL1 kua whakamahana i te 65°C i te wa e taea ai (ka rewa te paura o te whenua, ka tere te heke o te DNA ira tangata) .
4. Ko te rokiroki hee o Foregene Protease ka hua te heke, te kore mahi ranei.
Taunakitanga: Whakapumautia nga tikanga rokiroki o Foregene Protease, whakakapi ranei ki tetahi Foregene Protease hou mo te hauwai enzymatic.
5. Ko te kete kei te hee te penapena, te penapena ranei mo te roa rawa, ka taka etahi waahanga o te kete.
Taunakitanga: Hokona he kete tangohanga DNA genomic tipu hou mo nga mahi e pa ana.
6. He kino te whakamahi i te kete.
Huatau: Hokona he Kete Whakawehenga DNA Tipu kua whakatapua ki nga tauira mo te tangohanga me te purenga o te DNA genomic tipu.
7. Purihia te WB me te kore e tapiri ahauwai waiwaro.
Taunakitanga: Me whakarite te taapiri i te rōrahi tika o te waihā pūmau ki te Buffer WB.
8. Karekau i tika te whakamakuku o te eluent ki runga i te kirikiri kirika.
Huatau: Tāpirihia te eluent kua wera i mua i te 65℃maturuturunga iho ki te waenganui o te kiriuhi kirikiri kirika, ka waiho ki te pāmahana rūma mo te 5 meneti hei whakanui ake i te pai o te elution.
Tangohanga kia whiwhi DNA genomic iti
1. Ko te tauira kei te hee te penapena, te penapena ranei mo te roa rawa, ka puta te paheketanga o te DNA genomic.
Taunakitanga: Whakaorangia nga tauira kiko ki te -20℃;ngana ki te whakamahi i nga tauira kikokiko hou i kohia mo te tangohanga DNA ira.
2. Mēnā he iti rawa te rahi o ngā tauira kiko, ka iti ake te DNA genomic kua tangohia.
He whakaaro: He nui te wai o etahi tauira tipu, penei i nga tipu wai penei i te pukohu, me etahi atu, ka taea te whakanui tika i te inenga, ka maroke ranei te wai i mua i te mahi.
3. Ko nga tauira kaore i tino kuia ki te hauota wai, ka waiho ranei ki te pāmahana rūma mo te roa rawa i muri i te huri.
Huatau: Me tino rawaka te huri hauota wai, a me pakaru te pakitara o te tauira tauira ka taea;i muri tonu i te hurihanga, me whakawhiti te paura tauira ki te 65℃kua whakawerahia te Buffer PL1 mo te taahiraa e whai ake nei.
4. Kaua e whakamahi i te kete tika.
Taunakitanga: Whakamahia he Kete Wetewete DNA Tipu hei tango me te pure i te DNA ira tangata tipu.
5. Ko te rokiroki hee o te Foregene Protease ka heke, ka ngoikore ranei te mahi.
Taunakitanga: Whakapumautia nga tikanga rokiroki o Foregene Protease, whakakapi ranei ki tetahi Foregene Protease hou mo te hauwai enzymatic.
6. Raruraru pai
Taunakitanga: Tena koa whakamahia te Buffer EB mo te whakamaarama;ki te whakamahi ddH2O me etahi atu mea eluent, kia mohio kei waenganui i te 7.0-8.5 te pH o te eluent.
7. Kaore i te tika te whakamakuku o te eluent
Huatau: Taapirihia te maturuturutanga o te elution ki waenganui o te kirikiri kirika ka waiho ki te pāmahana rūma mo te 5 meneti hei whakanui ake i te pai o te elution.
8. He iti rawa te rōrahi eluent
Huatau: Tena koa whakamahia te eluent mo te whakangao DNA genomic kia rite ki nga tohutohu, kaua e iti iho i te 100μl.
Ko te DNA genomic kua tangohia he iti te parakore
Ko te iti o te parakore o te DNA ira ka arai ki te kore, ki te ngoikore ranei o nga whakamatautau o raro, penei: kaore e taea te tapahi i te enzyme, kaore e taea e te PCR te kongakonga ira whainga.
1. Te pokenga pūmua rereke, te poke RNA.
Tātaritanga: Kāre i whakamahia te Buffer PW ki te horoi i te tīwae;Kaore i whakamahia te Buffer PW ki te horoi i te pou i te tere o te centrifugation tika.
Huatau: ngana ki te whakarite kia kore he uanga i roto i te putorino i te wa e tukuna ana te supernatant i roto i te pou;kia tino horoia te pou purenga me te Buffer PW kia rite ki nga tohutohu, a kaore e taea te whakakore i tenei taahiraa.
2. Parahanga katote poke.
Tātaritanga: Ko te tīwae horoi Buffer WB i waihotia, i horoia kotahi noa iho ranei, ka puta he toenga katote.
Taunakitanga: Me horoi kia rua ki te Buffer WB kia rite ki nga tohutohu hei tango i nga toenga katote ka taea.
3. Te poke RNase.
Tātaritanga: Ko te RNase Exogenous ka taapirihia ki te Buffer;Ko te mahi horoi hee i roto i te Buffer PW ka puta he RNase toenga ka pa ki nga mahi whakamatautau RNA o raro, penei i te tuhi in vitro.
Huatau: Ka taea e nga kete tangohanga waikawa karihi Foregene te tango i te RNA me te kore he RNase taapiri, me nga reagents katoa i roto i te Kete Whakawehenga DNA Plant kaore e hiahiatia he RNase;kia tino horoia te pou purenga me te Buffer PW kia rite ki nga tohutohu, a kaore e taea te whakakore i tenei taahiraa.
4. Nga toenga waihano.
Tātaritanga: Whai muri i te horoinga o te tīwae purenga me te Buffer WB, kaore i mahia te centrifugation ngongo kau.
Taunakitanga: Whaia nga tohutohu mo te centrifugation ngongo kau.
Pukapuka Tohutohu:
Te Ahu Tohutohu Kete Wetewete DNA tipu
