Toto SuperDirectᵀᴹ PCR Kit-EDTA Blood Direct PCR Master Mix mo te Genotyping o te Toto
Whakaahuatanga kete:
Whakamahi tika i te EDTA anticoagulated toto katoa hei tauira mo te whakakaha PCR, he nui te tairongo me te kaha whakakaha teitei.
◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui, kaore he maimoatanga o mua, me te whakamahi tika i te toto katoa hei tauira PCR.
◮He kaha te kaha ki te whakakaha ake i te punaha, a ka taea e ia te kite i te ira ira me nga kongakonga DNA whainga kee i roto i te toto.
◮2× SuperEasyTMWhakaranu-EDTA Te manawanui o te toto: tae atu ki te 45% i roto i te toto tangata me te 20% i te toto kiore.
◮Kua kapi katoa te tauira, no reira kaore he take e maaharahara mo te pokenga tauira me nga hua PCR pai.
Whakatakotoranga
200 ×20μl rxns, 2000 ×20μl rxns
2× SuperEasyTMWhakaranu-EDTA: Kei roto te D-Taq DNA Polymerase, dNTPs, MgCl2, tauhohenga parepare, PCR tauhohenga whakarei, optimizer me te stabilizer, me era atu.2O ki te 2× SuperEasyTMWhakaranu-EDTA hei whakamahi i te tauhohenga PCR.
6× DNA Loading Buffer: Karekau he SDS i roto i te Buffer Loading.E taunaki ana ki te whakamahi i te 6× DNA Loading Buffer i whakauruhia ki te kete i te wa e mahi ana i te electrophoresis gel agarose kia whiwhi ai i nga hua electrophoresis pai.Kaua e whakamahi i te Utatanga Buffer kei roto SDS, ki te kore ka nui te hiku o te rama kanapa i roto i te huarahi i te wa o te electrophoresis, ka pa ki nga hua whakamatautau.
Nga waahanga kete
| 2× SuperEasyTMWhakaranu-EDTA |
| 6× DNA Loading Buffer |
| Tohutohu |
Nga waahanga me nga painga
-Kare he hiahia mo te purenga DNA e pau ana te wa me te utu nui, kaore he maimoatanga o mua, me te whakamahi tika i te toto katoa hei tauira PCR.
-He kaha te kaha whakakaha o te punaha, a ka taea e ia te kite i nga wahanga DNA i roto i te toto.
-2× SuperEasyTMWhakaranu-EDTA Te manawanui o te toto: tae atu ki te 45% i roto i te toto tangata me te 20% i te toto kiore.
-Ko te tauira kua kapi katoa, na reira kaore he take e awangawanga mo te pokenga tauira me nga hua PCR pai.
Te tono kete
I whakatapua ki te tautuhi PCR tika mo te EDTA anticoagulated toto katoa.(Tae atu: toto tangata, toto kiore, toto heihei, toto manu, toto kau, toto kuri, etc.)
Rerengamahi
Rokiroki me te ora o te Papa
Me penapena tenei kete ki te -20°C.
2× SuperEasyTMWhakaranu-EDTA, ki te whakamahia auau, ka taea hoki te penapena ki te 4 ℃ mo te rokiroki mo te wa poto (whakamahia i roto i nga ra 10).
6× DNA Loading Buffer, ka taea te penapena i te 4°C ranei -20°C mo te wa roa.
