Toto SuperDirectᵀᴹ PCR Kit(UNG)-EDTA Toto Direct PCR Master Mix mo te Whakatauira Toto
Whakatakotoranga
200 ×20μl rxns, 2000 ×20μl rxns
2× SuperEasyTMWhakaranu(UNG)-EDTA :I runga i te 2×SuperEasyTMWhakaranu-EDTA, ka whakamahia te dUTP hei utu mo te dTTP, a ko te enzyme UNG ka taea te whakaheke i te tauira kei roto te dUTP ka taapirihia i te wa ano, ka taea te aukati i te poke o nga hua whakakaha PCR.Kei roto ko te D-Taq DNA Polymerase, dNTPs, MgCl2, te parepare tauhohenga, te whakarei tauhohenga PCR, te arotau me te whakapumau, me era atu. I roto i te tauhohenga PCR, me tapiri noa atu te toto katoa e tika ana mo te anticoagulated, primers, me te ddH2O ki te 2× SuperEasyTMWhakaranu(UNG)-EDTA hei whakamahi i te tauhohenga PCR.
6× Te Puawai Uta DNA:Karekau he SDS i roto i te Buffer Uta.E taunaki ana ki te whakamahi i te 6× DNA Loading Buffer i whakauruhia ki te kete i te wa e mahi ana i te electrophoresis gel agarose kia whiwhi ai i nga hua electrophoresis pai.Kaua e whakamahi i te Utatanga Buffer kei roto SDS, ki te kore ka nui te hiku o te rama kanapa i roto i te huarahi i te wa o te electrophoresis, ka pa ki nga hua whakamatautau.
Nga waahanga kete
2× SuperEasyTMWhakaranu(UNG)-EDTA |
6× DNA Loading Buffer |
Tohutohu |
Nga waahanga me nga painga
- Kaore he hiahia mo te purenga DNA e pau ana te wa me te utu nui, kaore he maimoatanga o mua, me te whakamahi tika i te toto katoa hei tauira PCR.
-He kaha te kaha whakakaha o te punaha, a ka taea e ia te kite i nga wahanga DNA i roto i te toto.
-2× SuperEasyTMKo te Mix(UNG)-EDTA he nui te manawanui o te toto: tae atu ki te 45% ki te toto tangata me te 20% ki te toto kiore.
-Ko te tauira kua kapi katoa, na reira kaore he take e awangawanga mo te pokenga tauira me nga hua PCR pai.Pūnaha PCR anti-parahanga 2×SuperEasyTMWhakaranu (UNG) -EDTA, ka whakakore pai i te parahanga na nga hua PCR, me te whakarite i te motuhake me te tika o te whakanui.
Te tono kete
I whakatapua ki te tautuhi PCR tika mo te EDTA anticoagulated toto katoa.(Tae atu: toto tangata, toto kiore, toto heihei, toto manu, toto kau, toto kuri, etc.)
Rerengamahi
Rokiroki me te ora o te Papa
Ka penapenahia tenei kete ki te -20°C.
2× SuperEasyTMWhakaranu(UNG)-EDTA,Ki te whakamahia auau, ka taea hoki te penapena ki te 4°C mo te rokiroki mo te wa poto (whakamahia i roto i nga ra 10).
6× DNA Loading Buffer, Ka taea te penapena i te 4°C ranei -20°C mo te wa roa.