Toto SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Toto Direct PCR Master Mix mo te Genotyping o te Toto
Whakaahuatanga kete:
Whakamahi tika i te heparin anticoagulated toto katoa hei tauira mo te whakakaha PCR, he nui te tairongo me te kaha o te whakakaha.Kua taapirihia te punaha anti-pollution UNG hei aukati i nga hua pai.
◮He kaha te kaha ki te whakakaha ake i te punaha, a ka taea e ia te kite i te ira ira me nga kongakonga DNA whainga kee i roto i te toto.
◮2× SuperEasyTMWhakaranu(UNG)-Heparin He nui te manawanui o te toto: tae atu ki te 45% ki te toto tangata me te 20% ki te toto kiore.
◮Kua kapi katoa te tauira, no reira kaore he take e maaharahara mo te pokenga tauira me nga hua PCR pai.Pūnaha PCR anti-pollution
◮2×SuperEasyTMWhakaranuhia (UNG), te whakakore i te parahanga na nga hua PCR, me te whakarite i te motuhake me te tika o te whakanui.
Whakatakotoranga
200 ×20μl rxns, 2000 ×20μl rxns
2× SuperEasyTMWhakaranu(UNG)-Heparin: I runga i te 2× SuperEasyTMKo te Mix-Heparin, ka whakamahia te dUTP hei utu mo te dTTP, a ko te enzyme UNG ka taea te whakaheke i te tauira dUTP ka taapirihia i te wa ano, ka taea te aukati i te poke o nga hua whakakaha PCR.Kei roto ko te D-Taq DNA Polymerase, dNTPs, MgCl2, te parepare tauhohenga, te whakarei tauhohenga PCR, te arotau me te whakapumau, me era atu. I roto i te tauhohenga PCR, me tapiri noa atu te toto katoa e tika ana mo te anticoagulated, primers, me te ddH2O ki te 2× SuperEasyTM Mix(UNG)-Heparin e hāngai ana hei whakamahi i te tauhohenga PCR.
6× DNA Loading Buffer: Karekau he SDS i roto i te Buffer Loading.E taunaki ana ki te whakamahi i te 6× DNA Loading Buffer i whakauruhia ki te kete i te wa e mahi ana i te electrophoresis gel agarose kia whiwhi ai i nga hua electrophoresis pai.Kaua e whakamahi i te Utatanga Buffer kei roto SDS, ki te kore ka nui te hiku o te rama kanapa i roto i te huarahi i te wa o te electrophoresis, ka pa ki nga hua whakamatautau.
Nga waahanga kete
| 2× SuperEasyTMWhakaranu(UNG)-Heparin |
| 6× DNA Loading Buffer |
| Tohutohu |
Nga waahanga me nga painga
-He kaha te kaha ki te whakakaha ake i te punaha, a ka taea e ia te kite i te ira ira me nga kongakonga DNA whainga kee i roto i te toto.
-2× SuperEasyTMWhakaranu(UNG)-Heparin He nui te manawanui o te toto: tae atu ki te 45% ki te toto tangata me te 20% ki te toto kiore.
-Ko te tauira kua kapi katoa, na reira kaore he take e awangawanga mo te pokenga tauira me nga hua PCR pai.Pūnaha PCR anti-parahanga 2×SuperEasyTMWhakaranuhia (UNG), te whakakore i te parahanga na nga hua PCR, me te whakarite i te motuhake me te tika o te whakanui.
Te tono kete
I whakatapua ki te tautuhi PCR tika mo te Heparin anticoagulated toto katoa.(Tae atu: toto tangata, toto kiore, toto heihei, toto manu, toto kau, toto kuri, etc.)
Rerengamahi
Rokiroki me te ora o te Papa
Ko te tukanga katoa o te kawe pouaka hukapapa iti-mahana, ki te whakarite kei te noho te kete ki te <4 ℃.
2× SuperEasyTMMix(UNG)-Heparin, ki te whakamahia auau, ka taea hoki te rongoa ki te 4°C mo te rokiroki mo te wa poto (whakamahia i roto i nga ra 10).
6× DNA Loading Buffer, ka taea te penapena i te 4°C ranei -20°C mo te wa roa.
