EndoFree Maxi Plasmid Kit (Tīwae hurihuri)
Whakaahuatanga kete:
Ko te tukanga tangohanga katoa he 1 haora anake, me te whakarite i te watea me te tere o te mahi.
Whakaahuatanga
Ko te EndoFree Maxi Plasmid Kit e whakamahi ana i tetahi tikanga whakamaarama kirikiri kirika motuhake kia pai ai te here i te DNA plasmid.Ma te whakakotahi i te Endotoxin Removal Buffer P4 me te EndoFree Maxi Filtration Columns, ka taea te tango i te endotoxin, te pūmua me etahi atu poke.Ko te tukanga tangohanga katoa he 1 haora anake, me te whakarite i te watea me te tere o te mahi.
Te nui o te huakita e taunakitia ana mo ia mahi: Mo te plasmid-tiketike, e taunaki ana kia whakamahia te 100 ml o te paanui ahurea huakita, me te hua mai i te nuinga o te 500-1500 μg.Mo te plasmid iti-kape, 200 ml huakita pāpāho ahurea e tūtohu ana ki te whakaputa a tawhio noa 200-600 μg plasmids.
Nga waahanga kete
| Hua Waehanga | D909-E（10 whakaritenga） |
| Kaipupuri BL | 30 ml |
| Kaipupuri P1 | 100 ml |
| Kaipupuri P2 | 100 ml |
| Pūreirei P4 | 100 ml |
| Kaipupuri DW2 | 44 ml |
| Kaipupuri EB | 30 ml |
| RNase A(10 mg/ml) | 1 ml |
| DNAPure Maxi Spin Colums | 10 pcs |
| FinePure Maxi Filter Syringe | 10 pcs |
| 50 ml Ngongo Kohinga | 10 pcs |
Te kitenga o te Plasmid DNA Concentration me te Ma
Ko te ma me te kukū o te DNA plasmid kua tangohia ka kitea e te electrophoresis gel agarose me te spectrophotometer ultraviolet.Ko te uara OD260 o te 1 e rite ana ki te 50 μg/ml o te DNA pae rua.Ko te uara OD260/OD280 o te DNA plasmid kua purea ko te tikanga kei roto i te awhe o te 1.7-1.9.Ko te DNA plasmid kua purea ka taea te tono tika ki nga whakamatautau me nga whakaritenga parakore teitei, penei i te whakawhitinga pūtau, i roto ranei i nga whakamatautau vivo.
Te tono kete
Ko te DNA plasmid i tangohia mai i tenei kete ka taea te tono ki nga momo mahi o ia ra, tae atu ki te nakunaku enzymatic, PCR, te raupapa, te here, te hurihanga tae atu ki te whakawhiti mo nga momo momo pūtau.
Rokiroki me te ora o te Papa
EndoFree Maxi Plasmid Kit ka taea te penapena i te pāmahana rūma (15-25°C) i raro i nga tikanga maroke mo te 12 marama.Taapirihia te RNase A ki te Buffer P1 i mua i te whakamahinga tuatahi, uru pai ka rongoa ki te 2-8°C.Ka taea te rongoa i te ranunga mo te neke atu i te 6 marama.
