Ka whaia e matou te kaupapa whakahaere o "He mea motuhake te Kounga, Ko te Awhina te mea nui, Ko te ingoa te tuatahi", a ka tino hanga, ka tohatoha angitu ki nga kaihoko katoa mo te Kaituku Ipurangi Haina CE Whakaaetia te Waikawa Nucleic 48 Well Plate DNA/Rna Extraction Isolation Reagent Kit mo PCR Diagnostic Test, Mo etahi atu korero, tukuna mai he imeera ki a matou.Kei te titiro whakamua matou ki te mahi ki a koe.
Ka whai matou i te kaupapa whakahaere o "He mea motuhake te Kounga, Ko te Awhina te mea nui, Ko te ingoa te tuatahi", a ka tino hanga, ka tohatoha angitu ki nga kaihoko katoa moKete Wehenga Waikawa Nucleic Haina, Wehenga Waikawa Nucleic Viral, Nau mai ki te toro ki ta maatau kamupene, wheketere me to maatau whakaaturanga e whakaatu ana i nga momo taonga ka tutuki i to tumanako.I tenei wa, he pai ki te toro atu ki ta maatau paetukutuku, ka whakapau kaha a maatau kaimahi ki te tuku ratonga pai ki a koe.Kia mohio koe ki te whakapiri mai ki a maatau ki te hiahia koe ki etahi atu korero.Ko ta matou whainga he awhina i nga kaihoko ki te whakatutuki i o raatau whaainga.Kei te whakapau kaha matou ki te whakatutuki i tenei ahuatanga toa-toa.

Whakaahuatanga kete

50 Preps, 200 Preps

Ka whakamahia e tenei kete te pou miro me te tauira i whakawhanakehia e to taatau kamupene, ka taea te tango i te RNA katoa o te pure-pure me te kounga teitei mai i nga momo kararehe kararehe me te tino pai.Ka whakaratohia e ia he Tiwae Whakapai DNA-pai, ka taea te wehe me te whakauru i te DNA genomic mai i te supernatant me te kiko lysate, ngawari me te wa-whakaora;Ka taea e te Tiwae RNA-anake te here i te RNA ka taea te tukatuka i te wa kotahi me te tauira ahurei He maha nga tauira.

Ko te katoa o te punaha he RNase-Free, kia kore ai te RNA kua tangohia mai e whakaheke;Pūnaha Buffer RW1, Buffer RW2 Buffer washing system, kia kore ai te RNA i whiwhi i te pūmua, te DNA, te katote, me te parahanga pūhui waro.

Nga waahanga kete:

Nga waahanga me nga painga

■ Kaua e manukanuka ki te whakahekenga o te RNA;he RNase-Free te punaha katoa
■ Kia pai te tango i te Tiwae Horoi-ADN ma te whakamahi i te DNA
■ Tangohia te DNA me te kore e taapiri i te DNase
■ Maamaa-ka oti nga mahi katoa i te pāmahana rūma
■ Ka taea te mahi tere i roto i te 30 meneti
■ Haumaru-kare he reagent waro e hiahiatia ana
■ Te parakore teitei -OD260/280≈1.8-2.1

Te tono kete

He pai mo te tangohanga me te purenga o te RNA katoa mai i nga momo momo kopa kararehe hou, tio ranei, nga pūtau whakatipu.

Tawhā hua

■ Nga tono whakararo: te whenu tuatahi cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Tauira: kopa kararehe, pūtau whakatipu
■ Inenga: Tissues 10-20mg, Pūtau(2-5)×10⁶
■ Te kaha herenga DNA morahi o te pou purenga: 80 μg
■ Rōrahi Elution: 50-200 μl

Te rere mahi

Hoahoa

Kararehe Tapeke RNA Isolation Kit i rongoatia 20mg
Ko nga tauira kiore hou, tangohia te 5% RNA katoa kua purea 1% agar

Glycogel electrophoresis
1: Spleen 2: Tākihi
3: ate 4: Ngakau

Te rokiroki me te oranga o te papa

Ka taea te penapena i te kete mo te 24 marama ki te pāmahana rūma (15-25 ℃) 2-8 ℃ ranei mo te wa roa.Ka taea te penapena Buffer RL1 ki te 4 ℃ mo te 1 marama i muri i te taapiri i te β-mercapptoethanol (he kowhiri).

Ka whaia e matou te kaupapa whakahaere o "He mea motuhake te Kounga, Ko te Awhina te mea nui, Ko te ingoa te tuatahi", a ka tino hanga, ka tohatoha angitu ki nga kaihoko katoa mo te Kaituku Ipurangi Haina CE Whakaaetia te Waikawa Nucleic 48 Well Plate DNA/Rna Extraction Isolation Reagent Kit mo PCR Diagnostic Test, Mo etahi atu korero, tukuna mai he imeera ki a matou.Kei te titiro whakamua matou ki te mahi ki a koe.
Kaituku IpurangiKete Wehenga Waikawa Nucleic Haina, Wehenga Waikawa Nucleic Viral, Nau mai ki te toro ki ta maatau kamupene, wheketere me to maatau whakaaturanga e whakaatu ana i nga momo taonga ka tutuki i to tumanako.I tenei wa, he pai ki te toro atu ki ta maatau paetukutuku, ka whakapau kaha a maatau kaimahi ki te tuku ratonga pai ki a koe.Kia mohio koe ki te whakapiri mai ki a maatau ki te hiahia koe ki etahi atu korero.Ko ta matou whainga he awhina i nga kaihoko ki te whakatutuki i o raatau whaainga.Kei te whakapau kaha matou ki te whakatutuki i tenei ahuatanga toa-toa.