RT-PCR Easyᵀᴹ II(E Rua Hipanga)
Whakaahuatanga kete:
◮Ka taea te whakahaere tere me te tika te tātari ine ine o te RNA.
◮Ka whakamahia e te kete he reagent whakawhiti whakamuri motuhake a Foregene me te Foregene HotStar Taq DNA Polymerase i honoa me te punaha tauhohenga ahurei kia pai ake ai te kaha whakakaha me te motuhake o te tauhohenga.
◮ Ko te punaha tauhohenga kua arotauhia ka nui ake te maaramatanga o te tauhohenga, te kaha ake o te waiariki, me te pai ake o te manawanui.
Whakatakotoranga
RT-PCR ngawariTM II(E Rua Hipanga) kei roto nga reagents katoa mo te RT-PCR e rua-taahiraa, te whakakotahi i te tauhohenga RT me te tauhohenga PCR i roto i te kete kotahi, e whakarato ana i te RT Mix me te PCR Mix.Ka taea e te RT Mix ahurei te whakahiato i te cDNA whenu tuatahi-kounga teitei me te ngawari. 2× PCRMāmāTM WhakaranuApiti atu he tino kaha me te whakakaha motuhake.Ko te huinga pararopi o nga mea e rua ka kaha ake te kite i te ira e whai ana.
Nga waahanga kete
| RT-PCRMāmāTM II(E Rua Hipanga) | ||
| Kei roto kete | RT-02021 | RT-02022 |
| 100T(20μlpūnaha) | 500T(20μlpūnaha) | |
| 2× RT ngawariTM Whakaranu | 1ml | 1.7ml×3 |
| 2× PCRMāmāTM WhakaranuApiti atu | 1ml | 1.7ml×3 |
| Pūmatua tupurangi (50μM) | 200μl | 1ml |
| Oligo(dT)18 Primer (50μM) | 200μl | 1ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml |
| Pukapuka Tohutohu | 1wahi | 1wahi |
Nga waahanga me nga painga
-Ka taea te whakahaere tere me te tika te tātari ine ine o te RNA.
-Ka whakamahia e te kete he reagent whakawhiti whakamuri motuhake a Foregene me te Foregene HotStar Taq DNA Polymerase i honoa me te punaha tauhohenga ahurei kia pai ake ai te kaha whakakaha me te motuhake o te tauhohenga.
-Ko te punaha tauhohenga kua arotauhia ka nui ake te maaramatanga o te tauhohenga, te kaha ake o te waiariki, me te pai ake o te manawanui.
Rerengamahi
Rokiroki me te ora o te Papa
1.Nga tikanga kawe waka
Ko te tukanga katoa o te kawe pouaka hukapapa iti-mahana, ki te whakarite kei te noho te kete ki te <4 °C.
2. Nga tikanga rokiroki
Ka penapena te kete ki te -20°C.Whakaorangia te hua i roto i te pouaka whakamahana mahana tonu i te -20°C i muri tonu i te whiwhinga.Mena he pai nga tikanga rokiroki,te kore ia hua e whakaiti i tetahi mahi i roto i te 1-tau whaimana.
