Tika RT-qPCR Kit
Whakaahuatanga kete:
◮ Tika RT-qPCR Kit, ma te whakamahi i te Foreasy Reverse Transcriptase me te Foreasy HS Taq DNA Polymerase e whakawhanake ana e Foregene, me te aukati tauhohenga ahurei, ka hangaia tenei kete ki te kaha te aukati me te hototahi, a ka taea e ia te whakamatautau tika te whakamahi i te punaha Foregene Lysis hei tauira.Ka taea e tenei kete te tono mo te kete kimi waikawa nucleic COVID-19 me etahi atu huakita pathogenic ki te whanaketanga kete kitenga waikawa karihi.
Whakaahuatanga
Ko te Kete RT-qPCR Direct e whakarato ana i te parepare RT-qPCR motuhake me te whākōkī tino pairanunga (Taq, M-MLV, RI), kia watea mo te hanga o nga kete tuara me te punaha.whakatikatika，i roto i te manatoko ngawari.
Whakatakotoranga
500T, 50,000T
Nga waahanga kete
| Kei roto kete (20 μL pūnaha tauhohenga) | IM-05111-01 | IM-05112-01 |
| 500 T | 50,000 T | |
| Ranu Enzyme Tika | 500 μL×1 | 50 mL×1 |
| 2× Tika RT-qPCR Pūreirei | 1 mL×5 | 500 mL×1 |
| Tohutohu | 1 | 1 |
Nga Mahi Mahi
I mua i te takahanga reagent, me whakamakuku nga reagents katoa i roto i te kete ki te pāmahana rūma me te whakauru ata.
A: Whakaritea of tauira a reagent
1. Whakapaia te tauira RNA kua rite (E taunaki ana kia whakamahia te kete raupapa Kete Isolation RNA Tapeke o Foregene ki te tango me te pure i te tauira RNA) he tauira hua kapiti ranei (E taunaki ana kia whakamahi i te punaha Foregene Lysis), nga mea tuatahi (10 μM) me etahi atu taonga e tika ana, taputapu.
2. Whakanohoia te 2× Direct RT-qPCR Buffer, RNase-Free ddH2O me te 20× ROX Reference Dye(mehemea he's hiahiatia) i roto i te pouaka me te hukapapa, ka rewa noa enei, ka uru marie.
B: Whakaritea of RT-qPCR pūnaha
Ripanga 1 : Whakaritea of RT-qPCR system
| Waehanga | Rōrahi | Te kukū whakamutunga |
| 2× Tika RT-qPCR Pūreirei | 10 μL | 1× |
| Ranu Enzyme Tika | 1μL | |
| Pūmua Whakamua(10 μM) | 0.8 μL | 50-900nM |
| Pūmatua Whakamuri(10 μM) | 0.8 μL | 50-900nM |
| Probe(4 μM) | 1μL | 200nM |
| Tātauira(RNA, Lysate ranei) | X μL | |
| 20×ROX Waikano Tohutoro | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Rōrahi Tapeke | 20μL |
C: Tautuhi te RT-qPCR tauhohenga hōtaka
Ripanga 2 : RT-qPCR reaction hōtaka whakatakotoranga (mo tauira: rua-takahanga tikanga)
| Hipanga | Te pāmahana | Te wa | Nga huringa | Ihirangi | |
| 1 | 50 ℃ | 15 min | 1* | 1 | Whakamuri whakamuri |
| 2 | 95℃ | 1 min | 1 | Tuhinga o mua | |
| 3 | 95℃ | 10 hēkona | 40-45 | Te whakahekenga | |
| 60 ℃ | 30 hēkona 2* | Anaa/Torohanga | |||
Rokiroki me te ora o te Papa
-Me penapena te kete ki te -20± 5℃.Ko te wa mana he 12 marama.
-Katihia nga huringa whakatio-rewa (<5 huringa).
