ForeDirect RT-qPCR Kit
Whakaahuatanga kete:
◮Mo te RNA te whakakaha i te waa-a-waa mai i nga kohinga swab kaore he tukanga purenga RNA.Ka oti i tenei kete nga huringa qRT-PCR i roto i te haora kotahi.Ko te ranunga enzyme he whakakotahitanga papai o Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Kei roto i te Reaction Buffer nga waahanga katoa e hiahiatia ana, tae atu ki nga waahanga aukati kua arotauhia, Mg2+, dUTP, me nga dNTP.
Whakaahuatanga
Ko te ForeDirect RT-qPCR Kit he mea hoahoa mo te whakakaha tika o te RNA i te waa-a-waa mai i nga kohinga swab kaore he tikanga purenga RNA.Ka oti i tenei kete nga huringa qRT-PCR i roto i te haora kotahi.Ko te ranunga enzyme he whakakotahitanga papai o Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Kei roto i te Reaction Buffer nga waahanga katoa e hiahiatia ana, tae atu ki nga waahanga aukati kua arotauhia, Mg2+, dUTP, me nga dNTP.
Whakatakotoranga
100T
Nga waahanga kete
| He kaihoko tuku waikawa nucleic |
| RNA tiaki |
| 2× RT-qPCR Kaipupuri |
| Ranu Enzyme(Taq&M-MLV) |
| Tohutohu |
Taupānga PCR
1. Kia poto nga ngongo tauhohenga PCR, tuu ki roto i te tauira tank o te taputapu whakakaha.
2. Kawa wera
| Hipanga | Te pāmahana | Te wa | Nga huringa | |
| 1 | Whakamuri whakamuri | 50 ℃ | 15 min | 1 |
| 2 | Tuhinga o mua | 95℃ | 1 min | 1 |
| 3 | Te whakahekenga | 95℃ | 10 hēkona | 42 |
| 4 | Anaa/Torohanga | 60 ℃ | 30 hēkona | |
Tuhipoka:I kitea te tohu kareoro i muri tonu i te taahiraa toronga o ia huringa.
3. I muri i te tautuhi, tiakina te konae me te whakahaere i te kaupapa tauhohenga.
Nga Mahi Mahi
I mua i te takahanga reagent, me whakamakuku nga reagents katoa i roto i te kete ki te pāmahana rūma me te whakauru ata.
Te whakarite reagent
1. Whakaritea te Whakaranu Whakarewa Nucleic Acid
| Waehanga | Rōrahi mo ia Tauira, Mana ranei |
| He kaihoko tuku waikawa nucleic | 5μL |
| RNA tiaki | 0.5 μL |
2. Whakaritea te Whakaranu Tauhohenga
| Waehanga | Rōrahi mo ia Tauira, Mana ranei |
| 2× RT-qPCR Kaipupuri | 15 μL |
| Ranu Enzyme(Taq&M-MLV) | 1.5 μL |
| Primers and Probes | 1μL |
Rokiroki me te ora o te Papa
-Me penapena te kete ki te -20± 5℃.Ko te wa mana he 12 marama.
-Katihia nga huringa whakatio-rewa (<5 huringa).
