RT-PCR Easyᵀᴹ I(Kotahi Hipanga)
Whakaahuatanga kete:
◮Ma te kete kotahi-taahiraa ka taea te whakawhiti whakamuri me te PCR ki te ngongo kotahi.Me taapiri noa i te tauira RNA, nga tohu tuatahi PCR me te RNase-Free ddH2O.
◮Ka taea te whakahaere tere me te tika te tātari ine ine o te RNA.
◮Ka whakamahia e te kete he reagent whakawhiti whakamuri motuhake a Foregene me te Foregene HotStar Taq DNA Polymerase i honoa me te punaha tauhohenga ahurei kia pai ake ai te kaha whakakaha me te motuhake o te tauhohenga.
◮Ko te punaha tauhohenga kua arotauhia ka nui ake te maaramatanga o te tauhohenga, te kaha ake o te waiariki, me te pai ake o te manawanui.
Whakatakotoranga
Foregene One-Step RT-PCR EasyTM Ko nga hua raupapa e mohio ana ki te tauhohenga whakauru mai i te RNA ki te DNA paerua-rua, ara, ko te whakamaoritanga whakamuri me te whakakaha PCR kua oti i roto i te ngongo centrifuge tauhohenga me te punaha tauhohenga ano, e whakamaarama ana i nga mahi whakamatautau., arotau anathe kaupapa whakamatautau, mewhakapai ake te whakamatautau whai huatanga.
RT-PCR ngawariTM I(Kotahi Hipanga) he kete RT-PCR kotahi-taahiraa i hangaia e Foregene, e mohio ana ki te mahi tonu mai i te RT ki te PCR i roto i te ngongo kotahi.He ngawari, he tere hoki te mahi, ka taea te whakaiti i te parahanga i te wa o te whakamatautau.2×RT-PCR ngawariTM He kaha te manawanui o te ranunga, te pumau o te waiariki, te tino pai me te motuhaketenei mo whakakaha.
Nga waahanga kete
| RT-PCR ngawariTMAhau(Kotahi Hipanga) | ||
| Kei roto kete | RT-02011 | RT-02012 |
| 100T (20μl pūnaha) | 500T (20μl pūnaha) | |
| 2× RT-PCR HewariTM Whakaranu | 1ml | 1.7ml×3 |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Pukapuka Tohutohu | 1 wahi | 1 wahi |
Nga waahanga me nga painga
- Ma te kete kotahi-taahiraa ka taea te whakawhiti whakamuri me te PCR ki roto i te ngongo kotahi.Me taapiri noa i te tauira RNA, nga tohu tuatahi PCR me te RNase-Free ddH2O.
- Ka taea te tere me te tika te mahi tātari ine ine o te RNA.
- Ka whakamahia e te kete he reagent whakawhiti whakamuri motuhake a Foregene me te Foregene HotStar Taq DNA Polymerase i honoa me te punaha tauhohenga ahurei kia pai ake ai te kaha whakakaha me te motuhake o te tauhohenga.
- Ko te punaha tauhohenga kua arotauhia ka nui ake te maaramatanga o te tauhohenga, te kaha ake o te waiariki, me te pai ake o te manawanui.
Rerengamahi
Rokiroki me te ora o te Papa
1.Nga tikanga kawe waka
Ko te tukanga katoa o te kawe pouaka hukapapa iti-mahana, ki te whakarite kei te noho te kete ki te <4 °C.
2. Nga tikanga rokiroki
Ka penapena te kete ki te -20°C.Whakaorangia te hua i roto i te pouaka whakamahana mahana tonu i te -20°C i muri tonu i te whiwhinga.Mena he pai nga tikanga rokiroki,te kore ia hua e whakaiti i tetahi mahi i roto i te 1-tau whaimana.
