China Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish) Kaihanga me te Kaiwhakarato |Foregene

Kete PCR Tika Tiaki Zebra-UNG Direct PCR Reagent Raihana(zebrafish)

Kete PCR Tika Tiaki Zebra-UNG Direct PCR Lysis Reagent(zebrafish) Atahanga Whakaatu

Whakaahuatanga kete:

Ko te whakamahi tika i te lysate zebrafish hei tauira mo te whakakaha PCR, kaore he tangohanga motuhake o te DNA, he tere me te tairongo teitei.Kua taapirihia te punaha anti-parahanga UNG ki te whakakore i nga hua pai.

◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

◮He iti te tono tauira, me tango he kakano kotahi.

◮Kaore e hiahiatia nga maimoatanga motuhake penei i te huri me te kuru, a he ngawari te mahi.

◮Ko te punaha PCR kua arotauhia ka taea e te PCR te whai kiko ake me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

Tukuna he imeera ki a matou

Taipitopito Hua

Tohu Hua

Whakaahuatanga

Ka whakamahia e tenei kete he punaha lysis buffer motuhake kia tere te tuku i te DNA ira mai i te ika zebrafish me etahi atu kopa ika wai maori, he tara hiku, he tauira hua hua ika ranei mo nga tauhohenga PCR, no reira he tino pai mo te whakamatautau ira-nui.Ko te tukanga o te tuku i te DNA genomic mai i te lysis buffer ka oti i roto i te 10-30 meneti i te 65°C.Karekau he tukanga pera i te tango i te pūmua me te RNA e hiahiatia ana, ka taea te whakamahi te tohu DNA hei tauira mo te tauhohenga PCR.

Ko te 2× PCR EasyTM Mix (UNG) ka whakamahi i te dUTP hei utu mo te dTTP i runga i te 2× PCR EasyTM Mix, me te taapiri i te UNG enzyme (Uracil-N-glycosylase) ka taea te whakaheke i te tauira kei roto i te dUTP i te wa ano.I mua i te tauhohenga PCR, ka whakamahia te enzyme UNG ki te whakaheke i te hua PCR kei roto te uracil.Karekau he paanga o te UNG enzyme ki te tauira karekau he uracil, na reira ka whakarite i te motuhake me te tika o te whakakaha me te aukati i te tupono o te whakamatautau ira-nui.Kua pokea nga hua PCR.

Ko te D-Taq DNA polymerase he DNA polymerase i hangaia e Foregene mo nga tauhohenga PCR tika.Ko te D-Taq DNA polymerase he kaha te manawanui ki nga momo aukati tauhohenga PCR, a ka taea e ia te whakanui i nga tohu o te DNA i roto i nga momo punaha tauhohenga uaua, a ka eke te tere whakakaha ki te 2Kb/min, he mea tino pai mo te tauhohenga PCR Tika.

Whakatakotoranga

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nga waahanga kete

Wāhanga I	Kaipupuri FP
	Foregene Protease
	6× DNA Loading Buffer
Wahi II	2×PCR ngawari^TMWhakaranu(UNG)
Tohutohu

Nga waahanga me nga painga

■ Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

■ He iti te tono tauira, ka taea te whakamatautau i te 1mg o te hiwi caudal ika me te 10 nga hua ika.

■ Kaore e hiahiatia nga maimoatanga motuhake penei i te huri me te kuru, a he ngawari te mahi.

■ Pūnaha PCR Anti-pollution 2× PCR Easy TM Mix (UNG), ka taea te whakakore i te parahanga i puta mai i nga hua PCR me te whakarite i te motuhake me te tika o te whakanui.

■ Hangaia e te punaha PCR kua arotauhia te ahua o te PCR me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

Tawhā kete

Te Whakamahinga: Te ika Zebra me etahi atu ika wai hou.

Ko te DNA i tukuna mai i te tauira lysis: whakamahia anake hei tauira PCR.

Ka taea te whakamahi i te kete mo nga kaupapa e whai ake nei: te tautuhi i te transgenosis, te whakapapa kararehe, aha atu.

Rerengamahi

Rokiroki me te ora o te Papa

Ko te waahanga tuatahi o tenei kete me penapena ki te 2-8 ℃.

Ko te reagent Buffer FP ka taea te penapena mo nga marama 12 i raro i nga tikanga maroke;ka taea te penapena ki te 2-8 ℃ mo te rokiroki roa.

v Reagent Foregene Protease he tātai ahurei.Hei whakarite i tana mahi me te pumau, tiakina ki te 4 ℃ mo te 12 marama.

vReagent 6×DNA Loading Buffer ka taea te penapena i te 4 ℃, -20 ℃ ranei mo te wa roa.

Ko te wahanga II o tenei kete me pupuri ki te -20 ℃.

vReagent 2×PCR EasyTM Mix(UNG), mena ka whakamahia i nga wa katoa, ka taea hoki te penapena ki te 4 ℃ mo te rokiroki mo te wa poto (whakamahia i roto i nga ra 10).

Tōmua: Kete PCR Tika Tiaki Zebra Fish Reagent Reagent PCR Tika (zebrafish)

Panuku: Te Hiko Kiore Kete PCR Tupato Tuturu PCR Reagent Raihana(Tae Kiore)(mo te Tohuaira)

Tuhia to korero ki konei ka tukuna mai ki a matou