Foregene DNA Identification System 20A (Kore Te Tangohanga DNA)
Whakaahuatanga
Ko te punaha tohu DNA Foregene 20A e whakamahi ana i te hangarau tapanga korikori e rima-tae ki te whakanui i te 19 STR loci me te 1 loci ira i roto i te ngongo kotahi.I a ia e hipoki ana i nga waahi matua 13 CODIS, e 20 nga waahi matua e tutuki ana i nga whakaritenga a te Manatu mo te Haumarutanga a te Katoa mo te kete STR autosomal.Ko ia te ringa matau o te hanga papaa raraunga DNA forensic me te whakawhanaungatanga whakawhanaungatanga.
Ihirangi kete
Kete Waehanga | Ingoa Waehanga | Rōrahi(μl/ngongo) | Rahi (ngongo) |
PCR Reagent Tuhinga o mua | 2.5×PCR tauhohenga parepare Ⅲ | 1000 | 2 |
5×20A ranunga tuatahi | 500 | 2 | |
Mana DNA 9947A(1ng/μl) | 25 | 1 | |
Te wai whakaheke | 1700 | 2 | |
Taq Polymerase | Taq polymerase Ⅲ | 40 | 2 |
PCR Rear-reagent reagent | Allelic arawhata20A | 40 | 1 |
Paerewa o roto ORG 500 | 150 | 2 | |
Tuhipoka |
Tātari Raraunga
1. Whakatuwherahia te rorohiko Genemapper ID.Ka whakamahi koe i tenei kete mo te wa tuatahi, me kawemai e koe nga panui, huinga ipu, tikanga tātari e rite ana, me te paerewa rahi (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 30, 30, 30, 30, 30 0, 490, 500)
2. Kawemai i nga raraunga electrophoresis, tīpakohia nga tawhā tātari e hāngai ana penei i te paewhiri, te tikanga tātari, me te paerewa rahi, ka huri i te momo tauira o te arawhata ki te "Allelic Ladder" i te rarangi "Momo Tauira";timata te tātari raraunga.
Rokiroki Reagent
1. I muri i te whiwhinga i te kete tio i roto i te tio maroke, i nga kete tio kirihou ranei, ki te kore e whakamahia mo te wa poto, me penapena ki raro -20°C mo te wa roa.
2. I muri i te tangohanga o te kete me te whakamahi, me penapena te kete ki te 4°C i mua i te tauhohenga kia kore ai e toia te tio me te rewa;Me penapena te enzyme Taq ki te -20°C.
3. I muri i te tauhohenga, me penapena te kete (tae atu ki te taumaha o te ngota o roto me te arawhata) ki roto i te "ruma kimi hiko" i te 4°C, karohia te tio me te whakamaroke tonu, me te karo i te whakapiri atu ki te kete i mua i te tauhohenga kia kore ai e poke.