Foregene DNA Identification System 25A-C (Case)
Whakaahuatanga kete:
Ka whakamahia e te Foregene DNA Identification System 25A-C te hangarau tapanga rewharewha e ono-tae ki te whakanui i te 23 autosomal STR loci, kotahi Amel loci, kotahi Yindel loci me e rua nga waahi whakahaere kounga o roto i te wa kotahi mo te whakanui i nga tauira DNA i tangohia ka purea mai i nga tauira keehi.
Whakaahuatanga
Ka whakamahia e te Foregene DNA Identification System 25A-C te hangarau tapanga rewharewha e ono-tae ki te whakanui i te 23 autosomal STR loci, kotahi Amel loci, kotahi Yindel loci me e rua nga waahi whakahaere kounga o roto i te wa kotahi mo te whakanui i nga tauira DNA i tangohia ka purea mai i nga tauira keehi.
Ihirangi kete
Whakaritenga punaha whakakaha
Te Whakaritenga Whakaritenga mo te tangohanga Paerewa me te punaha whakakaha
| Waehanga | Pūnaha 10μl(μl) | |
| Whakaranu Matua | 2.5×PCR Premix C Ⅰ | 4.0 |
| 10×25A-CPrimer Mix | 1.0 | |
| tauira DNA | 1—5 | |
| Te wai whakaheke | Hangaia he rōrahi tauhohenga o te 10μl |
Tātari Raraunga
Kauwhata Apiti 1:Te whakapapa o te arawhata o te allele
Kauwhata Apiti 2:ADN patopato paerewa 9948 patopatomahere whenua
Rokiroki Reagent
1. I muri i te whiwhinga o te kete, penapenahia ki raro -20 ℃ ki te kore koe e whakamahi mo tenei wa.
2. I muri i te tangohanga o te kete ka whakamahia, ka rongoahia te wairewa whakauru PCR i mua i te tauhohenga ki te -20 ℃, me te toenga o nga reagents i mua i te tauhohenga ka rongoa ki te 4 ℃ kia kore ai e tukurua te huka-rewa.Mēnā he iti te inenga kotahi, ka tūtohu kia penapena ki te -20°C i muri i te tākainga.
3. Whai muri i te tauhohenga, ka pupurihia nga waahanga ki te 4 ℃ kia kore ai e tukurua te huka-rewa.Kaua e pa ki nga reagents i mua i te tauhohenga ki te karo i te parahanga.
