Foregene DNA Identification System Y Plus ( Tangohanga-kore)
Whakaahuatanga kete:
ForegeneKo te punaha tautuhi DNA a Y Plus e whakamahi ana i te hangarau tapanga rewharewha tae-ono ki te whakanui i te 20 Y chromosome loci matua, 15 waahi pai, 6 waahi rereke me te 3 Y indel loci i te wa kotahi.
Whakaahuatanga
ForegeneKo te punaha tautuhi DNA a Y Plus e whakamahi ana i te hangarau tapanga rewharewha tae-ono ki te whakanui i te 20 Y chromosome loci matua, 15 waahi pai, 6 waahi rereke me te 3 Y indel loci i te wa kotahi.
Ihirangi kete
Whakaritenga punaha whakakaha
Ko tehanganga whakariteo stohurd(tango-kore)pūnaha whakakaha
| Components | 25 μlpūnaha (μl) | Pūnaha 10μl (μl) | |
| Whakaranu Matua | 4 × Kaiwhakaako MixVII | 6.25 | 2.5 |
| 5×Y Plus Primer Mix | 5.0 | 2.0 | |
| Te wai whakaheke | 13.75 | 5.5 | |
| Briu poke | 1.2mm te diameter | 1.2mm te diameter | |
| Trōrahi tauhohenga otal | 25 μl | 10 μl | |
Tātari Raraunga
Kauwhata Apiti 1:Mahere Pato Ataata Allelic
Kauwhata Apiti 2:ADN patopato paerewa 9948 patopatomahere whenua
Rokiroki Reagent
1. Tukuna koa ki raro -20°C(Ki te kore e whakamahia mo te wa poto), i muri i te whiwhinga o nga kete kua whakatotoka ki roto i te tio maroke, i nga kete tio reera ranei.
2. Pupuritia koa te wahanga o mua i te tauhohenga 4×premix VII ki te -20°C, i muri i te tangohanga o te kete mo te whakamahi, me era atu reagents i mua i te tauhohenga ka rongoa ki te 4°C kia kore ai e toia te toi me te rewa.Mena he iti te inenga kotahi, ka tūtohu kia penapena ki te -20 ° C i muri i te tuku.
3. Whakaorangia nga wahanga i muri i te tauhohenga ki te 4°C, karohia te tio me te whakamaroke tonu, kaua hoki e pa ki nga reagents i mua i te tauhohenga kia kore ai e poke.
