Foregene DNA Identification System 27Y
Whakaahuatanga kete:
Foregene Ko te Pūnaha Whakatau DNA 27Y e whakamahi ana i te hangarau tapanga korikori ono-tae ki te whakanui i te 27 Y-STR loci i te wa kotahi.Tae atu ki: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS537S, DYS393, DYS393, DYS393, DYS393 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
He pai te kaha o te kete ki te aukati i te aukati, a ka taea te whakakaha tika i te pepa tātari, te kaari FTA, te miro, te kaari huware, te horoi waha, ka taea hoki te whakamahi ki te tango me te pure i nga tauira DNA mai i nga rawa take.
Whakaahuatanga
Foregene Ko te Pūnaha Whakatau DNA 27Y e whakamahi ana i te hangarau tapanga korikori ono-tae ki te whakanui i te 27 Y-STR loci i te wa kotahi.Tae atu ki: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS537S, DYS393, DYS393, DYS393, DYS393 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
He pai te kaha o te kete ki te aukati i te aukati, a ka taea te whakakaha tika i te pepa tātari, te kaari FTA, te miro, te kaari huware, te horoi waha, ka taea hoki te whakamahi ki te tango me te pure i nga tauira DNA mai i nga rawa take.
Ihirangi kete
| He kete whakamatautau reagent | Ingoa wae | Rōrahi (μl/ngongo) | Rahi (ngongo) |
| PCR kete o mua tauhohenga | 2×PCR Reaction Master Mix | 1250 | 2 |
| 5 x 27Y Whakaranu Tuatahi | 500 | 2 | |
| Paerewa Pato DNA 9948 | 20 | 2 | |
| Te wai whakaheke | 1700 | 2 | |
| Kete Tauhohenga Pou-PCR | 27Y ranunga allele arawhata | 20 | 2 |
| ORG500 te taumaha ngota o roto karaka | 150 | 2 |
Tātari Raraunga
1. Whakatuwherahia te rorohiko Gene Mapper ID.Ina whakamahi koe i tenei kete mo te wa tuatahi, me kawemai koe i nga panui, huinga ipu, tikanga tātari e rite ana, me te paerewa rahi (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 30, 30, 30, 30, 30, 30 , 490, 500).
2. Kawemai i nga raraunga electrophoresis, tīpakohia nga tawhā tātari e hāngai ana penei i te paewhiri, te tikanga tātari, me te paerewa rahi, ka huri i te momo tauira o te arawhata ki te "Allelic Ladder" i te rarangi "Momo Tauira";timata ki te tātari i nga raraunga.
Rokiroki Reagent
1. Whakaorangia koa ki raro -20°C (mehemea karekau e whakamahia mo te wa poto), i muri i te whiwhinga o nga kete kua whakatotoka i roto i te tio maroke, i nga kete tio reera ranei.
2. Pupuritia koa te kete ki te 4°C i mua i te tauhohenga kia kore ai e tukurua te mīti me te rewa, i muri i te tangohanga o te kete hei whakamahi, ;me pupuri te whākōkī ki te -20°C.
2. Whai muri i te tauhohenga, me penapena te kete (tae atu ki te taumaha o te ngota o roto me te arawhata) ki roto i te "ruma kimi hikohiko" ki te 4°C, kia karohia te tio me te whakamaroke tonu, me te karo i te whakapiri ki te kete o mua tauhohenga kia kore ai e poke.
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