Foregene DNA Identification System 30A (Kaore i Tangohanga)
Whakaahuatanga kete:
FOREGENE DNA identification system 30A e whakamahi ana i te hangarau tapanga rewharewha e ono tae ki tewhakanuia 29autosomalSTR waahi, kotahiAmel locus me tetahi Yindel locus i te wa kotahi, ka taea te whakakaha tika i te pepa tātari, FTAkāri, miro miro, kaari huware,me te mpania waho.
Whakaahuatanga
FOREGENE DNA identification system 30A e whakamahi ana i te hangarau tapanga rewharewha e ono tae ki tewhakanuia 29autosomalSTR waahi, kotahiAmel locus me tetahi Yindel locus i te wa kotahi, ka taea te whakakaha tika i te pepa tātari, FTAkāri, miro miro, kaari huware,me te mpania waho.
Ihirangi kete
Whakaritenga Pūnaha Whakanui
Ripanga 1: Ko te hanganga takahanga o te tauhohenga whakakaha Paerewa (Tangohanga-kore).
|
| Components | 25 pūnaha μl (μl) | 10 pūnaha μl (μl) |
| Whakaranu Matua | 4 × Whakaranu Matua VII | 6.25 | 2.5 |
| 5 x 30AWhakaranu Tuatahi | 5.0 | 2 | |
| Te wai whakaheke | 13.75 | 5.5 | |
| Briu poke | 1.2mm te diameter | 1.2mm te diameter | |
| Trōrahi tauhohenga otal | 25 μl | 10 μl | |
Tātari Raraunga
Kauwhata Apiti 1:Mahere Pato Ataata Allelic
Kauwhata Apiti 2:ADN patopato paerewa 9948 patopatomahere whenua
Rokiroki Reagent
1. Whakaorangia koa ki raro -20°C,, i muri i te whiwhinga o nga kete kua whakatotoka ki roto i te tio maroke, i nga kete tio reera ranei.
2. Pupuritia koa te wahanga o mua i te tauhohenga 4×premix VII ki te -20°C, i muri i te tangohanga o te kete mo te whakamahi , ka penapena i etahi atu reagents i mua i te tauhohenga ki te 4°C kia kore ai e toia te toi me te rewa.Mena he iti te inenga kotahi, ka tūtohutia kia penapena ki te -20°C i muri i te tuku,
3. Whakaorangia nga wahanga i muri i te tauhohenga ki te 4°C, karohia te tio me te whakamaroke tonu, kaua hoki e pa ki nga reagents i mua i te tauhohenga kia kore ai e poke.
