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Toto DNA Mini Kit
Kia tere te pure i te DNA genomic-kounga teitei mai i te toto anticoagulated (<1ml).
◮Karekau he poke RNase:Ko te Tiwae DNA-anake e whakaratohia ana e te kete ka taea te tango i te RNA mai i te DNA genomic me te kore e taapiri i te RNase i te wa o te whakamatautau, kia kore ai e poke te taiwhanga e te RNase o waho.
◮Tere tere:He nui ake te mahi a Foregene Protease i nga momo porotease rite, a ka keri tere i nga tauira kiko;he ngawari te mahi, a ka taea te mahi tangohanga DNA genomic i roto i nga meneti 20-80.
◮Watea:Ka mahia te centrifugation i te pāmahana rūma, a kaore he hiahia mo te 4°C iti-mahana centrifugation me te waihanol precipitation o DNA.
◮Haumaru:Kaore he tangohanga reagent waro e hiahiatia ana.
◮Kounga teitei:Ko te DNA genomic kua tangohia he mahanga nui, kahore he RNA, kahore he RNase, he iti rawa te ihirangi katote, ka taea te whakatutuki i nga whakaritenga o nga momo whakamatautau.
◮Pūnaha moroiti:Ka taea e ia te whakanui ake i te kukū o te DNA genomic, he watea mo te rapunga o raro, te whakamatautau ranei.
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General Plasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kit
Kia tere te purei i te DNA plasmid kounga teitei mai i nga huakita kua huri mo nga whakamatautau koiora ngota ngota penei i te panoni me te nakunaku whākōkī.
◮Tangohia te RNA me te kore e taapiri i te RNase
◮Watea—ka mahia te centrifugation i te pāmahana rūma
◮Tere—ka taea te mahi i roto i te 20 meneti
◮Haumaru—kaore he reagent waro i whakamahia
◮Te parakore teitei—OD260/280≈1.7-1.9
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RT Easyᵀᴹ I Kaiwhakaako Premix mo te whenu tuatahi cDNA synthesis
- He pai te punaha tuhi whakamuri, he 25 meneti noa iho hei whakaoti i te whakahiatotanga o te whenu cDNA tuatahi.
- He punaha whakamuri whakamuri teitei, ka taea e nga tauira taumata-pg te tiki cDNA kounga teitei.
- Ko te punaha whakamuri whakamuri he nui te waiariki, te pāmahana tauhohenga o te punaha ka eke ki te 50 ℃,a he pai tana mahi tuhi whakamuri.
- Ahakoa nga tauira RNA poke (te waipiro tae atu ki te 45%, te tote guanidine tae atu ki te 750mM) ka tukuna ki te tauhohenga whakawhiti whakamuri.
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RT Easy II(me te gDNase) Master Premix mo te whenu tuatahi cDNA synthesis mo te PCR Wā Tuturu me te gDNase
-He kaha ki te tango i te gDNA, ka taea te tango i te gDNA i roto i te tauira i roto i te 2 meneti.
- He pai te punaha whakawhiti whakamuri, he 15 meneti noa iho hei whakaoti i te whakahiatotanga o te whenu tuatahi cDNA.
-Tairaira Matatini: ka taea hoki te huri i nga tauira me te GC teitei me te hanganga tuarua matatini me te tino pai.
-Ko te punaha whakamuri whakamuri teitei, ka taea e nga tauira taumata-pg te tiki cDNA kounga teitei.
-Ko te punaha tuhi whakamuri he nui te pumau o te waiariki, ko te pāmahana tauhohenga tino pai ko te 42℃, a he pai tonu te mahi whakawhiti whakamuri i te 50℃.
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Wā Tuturu PCR Easyᵀᴹ-SYBR Green I kete
Maama—2X PCR Mix hei whakaiti i te hapa whakamatautau me te wa mahi
Ka taea e te parepare kua tino arotautia me te wera-whana Taq enzyme te aukati i te whakakaha kore-motuhake me te hanga dimer tuatahi
Te tairongo teitei—ka kitea nga kape iti o te tauira
He pai te mahi—he hototahi ki te nuinga o nga taputapu PCR ine i te waa-tūturu
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Kararehe Tapeke RNA Isolation Kete Tapeke RNA Tangohanga me te purenga kete mo nga Tissue kararehe me te Pūtau
He tere me te whai hua te tango i te RNA tapeke tino parakore me te kounga teitei mai i nga momo kopa kararehe.
Kaore he take ki te awangawanga mo te whakahekenga o te RNA.Ko te punaha katoa he RNase-Kore
He pai te tango i te DNA ma te whakamahi i te Tiwae Horoi DNA
Tangohia te DNA me te kore e taapiri i te DNAase
He ngawari—ka oti nga mahi katoa i te pāmahana rūma
Tere—ka oti te mahi i roto i te 30 meneti
Haumaru—kaore he reagent waro i whakamahia
Te parakore teitei—OD260/280≈1.8-2.1
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Pūtau Tapeke RNA Wehewehenga Kete Tapeke RNA Wehewehenga Kete Pure mai i te pūtau
Ka taea te whiwhi RNA tapeke tino purea me te kounga teitei mai i nga momo momo tipu i roto i te 11 meneti.
RNase-Free
Ka whakahaerehia i te pāmahana rūma (15-25 ℃)
Me te Tiwae Whakapai DNA
Hua RNA teitei
Tere:Whakaotia te tangohanga i roto i te 11 meneti
Haumaru: Kore he matū pararopi
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Toto DNA Midi Kit (1-5ml) Toto Isolation Midi Kits mo te DNA mai i te Toto
Kia tere te pure i te DNA genomic kounga teitei mai i te toto anticoagulated (1-5ml).
◮Karekau he poke RNase:Ko te Tiwae DNA-anake e whakaratohia ana e te kete ka taea te tango i te RNA mai i te DNA genomic me te kore e taapiri i te RNase i te wa o te whakamatautau, kia kore ai e poke te taiwhanga e te RNase o waho.
◮Tere tere:He nui ake te mahi a Foregene Protease i nga momo porotease rite, a ka keri tere i nga tauira kiko;he ngawari te mahi, a ka taea te mahi tangohanga DNA genomic i roto i nga meneti 20-80.
◮Watea:Ka mahia te centrifugation i te pāmahana rūma, a kaore he hiahia mo te 4°C iti-mahana centrifugation me te waihanol precipitation o DNA.
◮Haumaru:Kaore he tangohanga reagent waro e hiahiatia ana.
◮Kounga teitei:Ko te DNA genomic kua tangohia he mahanga nui, kahore he RNA, kahore he RNase, he iti rawa te ihirangi katote, ka taea te whakatutuki i nga whakaritenga o nga momo whakamatautau.
◮Pūnaha moroiti:Ka taea e ia te whakanui ake i te kukū o te DNA genomic, he watea mo te rapunga o raro, te whakamatautau ranei.
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Puka Uwau/Kari FTA Kete Wetewete DNA Te Tangohanga DNA Genomic, Kete Purifiaction ranei mai i Buccal Swabs
Kia tere te pure i te DNA genomic-kounga teitei mai i nga tauira o te Kari kaukau/FTA.
◮Karekau he poke RNase:Ko te Tiwae DNA-anake e whakaratohia ana e te kete ka taea te tango i te RNA mai i te DNA genomic me te kore e taapiri i te RNase i te wa o te whakamatautau, kia kore ai e poke te taiwhanga e te RNase o waho.
◮Tere tere:He nui ake te mahi a Foregene Protease i nga momo porotease rite, a ka keri tere i nga tauira kiko;he ngawari te mahi, a ka taea te mahi tangohanga DNA genomic i roto i nga meneti 20-80.
◮Watea:Ka mahia te centrifugation i te pāmahana rūma, a kaore he hiahia mo te 4°C iti-mahana centrifugation me te waihanol precipitation o DNA.
◮Haumaru:Kaore he tangohanga reagent waro e hiahiatia ana.
◮Kounga teitei:Ko te DNA genomic kua tangohia he mahanga nui, kahore he RNA, kahore he RNase, he iti rawa te ihirangi katote, ka taea te whakatutuki i nga whakaritenga o nga momo whakamatautau.
◮Pūnaha moroiti:Ka taea e ia te whakanui ake i te kukū o te DNA genomic, he watea mo te rapunga o raro, te whakamatautau ranei.
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Foregene DNA Identification System 27Y
Foregene Ko te Pūnaha Whakatau DNA 27Y e whakamahi ana i te hangarau tapanga korikori ono-tae ki te whakanui i te 27 Y-STR loci i te wa kotahi.Tae atu ki: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS537S, DYS393, DYS393, DYS393, DYS393 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
He pai te kaha o te kete ki te aukati i te aukati, a ka taea te whakakaha tika i te pepa tātari, te kaari FTA, te miro, te kaari huware, te horoi waha, ka taea hoki te whakamahi ki te tango me te pure i nga tauira DNA mai i nga rawa take.
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Foregene DNA Identification System 38Y-C (Case)
Foregene Pūnaha tautuhi DNA 38Y-Ka whakamahi a C i te hangarau tapanga rewharewha e ono tae ki te whakanui i te 38 Y chromosome STR loci , 3 Y-Indel lociae rua nga waahi whakahaere kounga o roto i te wa kotahimo te whakakaha Nga tauira take i tangohia mai i nga tauira DNA kua purea.
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Foregene DNA Identification System 30A (Kaore i Tangohanga)
FOREGENE DNA identification system 30A e whakamahi ana i te hangarau tapanga rewharewha e ono tae ki tewhakanuia 29autosomalSTR waahi, kotahiAmel locus me tetahi Yindel locus i te wa kotahi, ka taea te whakakaha tika i te pepa tātari, FTAkāri, miro miro, kaari huware,me te mpania waho.
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