Tikanga RT-qPCR raupapa
PCR tika
He aha te Direct PCR
Ko te PCR tika te tikanga mo te whakakaha DNA tika mai i te toto, te kiko kararehe, te hiku kiore, te pūtau, te taringa kiore, te ika zebra, nga hua ika, nga rau tipu, nga kakano me etahi atu tauira kiko koiora taketake me te kore e mahi i te wehenga DNA me te purenga.Ka taea e te hangarau PCR tika te whakaiti i te wa whakamatautau, me te utu mo te mahi ira tangata me nga kaupapa nui.He pai ake ano te whiringa ina pa ana ki nga wero o te whakanui i te rahinga iti rawa o te tauira ka taea e te taahi purenga te mate tauira.
He kaha te awangawanga a FOREGENE's PCR Mix, na te kaha o FOREGENE i patented Taq enzyme( patent mana: ZL20161034512.0 (Haina), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)).Ka whakamahia e tana enzyme Taq te hangarau whakakotahi DNA ki te whakakotahi ano i te rohe hanganga here DNA o te pūmua here waikawa karihi archaeal me te Taq DNA polymerase-wera hei hanga i tetahi tauira DNA kua piki ake te hononga.He Taq timata-wera kua pai ake e pupuri ana i nga momo mahi o te DNA polymerase.
Hei tauira, he ngawari ki te taapiri i to pukoro, tipu (te rau taitamariki, te pakiaka, te kakano ranei) he tauira kararehe ranei ki te papaa, me te taapiri i te ranunga rangatira ahurei me nga mea tuatahi i roto i nga ngongo PCR me te whakahaere ma te miihini hiko.He pai te pai o te kete mo te tātari tauira nui-nui he mea tino nui te penapena wa.
Ko te punaha tauhohenga PCR kei roto ko te UNG enzyme me te dUTP ka tohua i runga i nga whakaritenga whakamatautau, ka taea te karo i te parahanga na nga hua whakakaha PCR.
Painga oDPCR tika
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| PCR tika | Tikanga tuku iho (Te purenga RNA+ PCR Tikanga) |
| Te kai rauemi | Te kohi kiko | Iti iho | Nui |
| Te whakarite tauira | Momo rauemi | Kaore e hiahiatia te huri | Te huri tauira |
| Mahi | Momo ngongo kotahi，10min | mahi uaua, 1h | |
| Rereke | 1-96 | 1-24 | |
| tauhohenga PCR | pūnaha tauhohenga | 2×PCR ranunga kua arotau | Dntp, MgCl2,10×PCR Buffer,Taq enzyme, |
Application
01 Te tautuhi i nga microorganisms pathogenic
02 Te tautuhi ira, te tohu ira
03 Multiplex PCR / SNP detection / PCR-RFLP
04Whakaraupapa/whakarakau
Raupapa Hua
| Rangatū | Ingoa Hua | Whakatakotoranga | Nama Putumōhio | Nga Tikanga Rokiroki |
| Tikanga RT-qPCR Series | 200T | DRT-01011 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 1000T | DRT-01012 |
| Rangatū | Ingoa Hua | Whakatakotoranga | Nama Putumōhio | Nga Tikanga Rokiroki |
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Toto Direct PCR Series | 200 ×20μl rxns | TP-04111 | -20 ℃ | |
| 2000 ×20μl rxns | TP-04113 | |||
| 200 ×20μl rxns | TP-04121 | -20 ℃ | ||
| 2000 ×20μl rxns | TP-04123 | |||
| 200 ×20μl rxns | TP-04211 | -20 ℃ | ||
| 2000 ×20μl rxns | TP-04213 | |||
| 200 ×20μl rxns | TP-04221 | -20 ℃ | ||
| 2000 ×20μl rxns | TP-04223 |
| Series | Ingoa Hua | Whakatakotoranga | Nama Putumōhio | Nga Tikanga Rokiroki |
| Kararehe Tika PCR Series | Kete PCR Tika Tiu Kararehe(65℃ lysis) | 200 ×20μl rxns | TP-01111 | Wāhanga I 4℃，Wāhanga II -20℃ |
| 2000 ×20μl rxns | TP-01113 | |||
| Kete PCR Tika Tiu Kararehe(65℃ lysis) -UNG | 200 ×20μl rxns | TP-01121 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-01123 | |||
| Hiku Kiore SuperDirectTMKete PCR (whakahekenga pāmahana rūma) | 200 ×20μl rxns | TP-01311 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-01313 | |||
| Kete PCR Hiku Tail Kiore (65℃ lysis) | 200 ×20μl rxns | TP-01331 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-01333 | |||
| Kiore Tail Direct PCR Kit(65℃ lysis) - UNG | 200 ×20μl rxns | TP-01341 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-01343 | |||
| Kete PCR Tika ika Zebra(65℃ lysis) | 200 ×20μl rxns | TP-01411 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-01413 | |||
| Zebra Fish Direct PCR Kit(65℃ lysis) - UNG | 200 ×20μl rxns | TP-01421 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-01423 |
| Rangatū | Ingoa Hua | Whakatakotoranga | Nama Putumōhio | Nga Tikanga Rokiroki |
| Rangatū PCR Tiaki Tiaki | 200 ×20μl rxns | TP-02111 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-02113 | |||
| 200 ×20μl rxns | TP-02121 | Wāhanga I 4℃，Wāhanga II -20℃ | ||
| 2000 ×20μl rxns | TP-02123 | |||
| Tipu Rau Direct PCR Plus Kit | 200 ×20μl rxns | TP-02131 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-02133 | |||
| Tipu Rau Direct PCR Plus Kit -UNG | 200 ×20μl rxns | TP-02141 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-02143 | |||
| 200 ×20μl rxns | TP-03111 | Wāhanga I 4℃，Wāhanga II -20℃ | ||
| 2000 ×20μl rxns | TP-03113 | |||
| 200 ×20μl rxns | TP-03121 | Wāhanga I 4℃，Wāhanga II -20℃ | ||
| 2000 ×20μl rxns | TP-03123 | |||
| 200 ×20μl rxns | TP-03131 | Wāhanga I 4℃，Wāhanga II -20℃ | ||
| 2000 ×20μl rxns | TP-03133 | |||
| 200 ×20μl rxns | TP-03141 | Wāhanga I 4℃，Wāhanga II -20℃ | ||
| 2000 ×20μl rxns | TP-03143 | |||
| Kakano Kakano Tiaki PCR Plus Kit I(Polysaccharide polyphenol tipu) | 200 ×20μl rxns | TP-03151 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-03153 | |||
| Kakano Kakano Tiaki PCR Plus Kit I - UNG(Polysaccharide polyphenol tipu) | 200 ×20μl rxns | TP-03161 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-03163 | |||
| 200 ×20μl rxns | TP-03171 | Wāhanga I 4℃，Wāhanga II -20℃ | ||
| 2000 ×20μl rxns | TP-03173 | |||
| Kakano Kakano Tika PCR Plus Kit II - UNG(Polysaccharide polyphenol tipu) | 200 ×20μl rxns | TP-03181 | Wāhanga I 4℃，Wāhanga II -20℃ | |
| 2000 ×20μl rxns | TP-03183 |
