Koiora Molecular Reagent Kaituku me te wheketere |Haina Nga Kaihanga Reagent Molecular Biology

Reagent Biology Molecular

RNA raupapa wehe

He raupapa wehewehe DNA

Tikanga RT-qPCR raupapa

Te raupapa PCR tika toto

Whakatokia raupapa PCR tika

He raupapa PCR tika kararehe

PCR/RT-PCR raupapa

IVD nga rauemi mata

Te aukati RNase Foreasy

◮He aukati RNase i ahu mai i te kiore hou e whakaatuhia ana i roto i nga huakita i hangaia e E. coli ma te whakamahi i te hangarau whakakotahi ira.Ka aukati te aukati i te mahi RNase ma te whakakotahi kore whakataetae me te RNase 1: 1, na reira ka tiakina.RNAte tapatahi, a ka taea te aukatiteRNase A, B, C mahi , engari ehara i te RNase T1, T2, H, me etahi atu; Ka taea te whakahuri o te here o RNase Inhibitor me RNase , a ka taea te wehewehe i te matatini e te urea me te sulfhydryl reagents, kia kore e taea te whakakore i te RNase renatures me te aukati.

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Kete PCR Tika Tiaki Zebra-UNG Direct PCR Reagent Raihana(zebrafish)

Ko te whakamahi tika i te lysate zebrafish hei tauira mo te whakakaha PCR, kaore he tangohanga motuhake o te DNA, he tere me te tairongo teitei.Kua taapirihia te punaha anti-parahanga UNG ki te whakakore i nga hua pai.

◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

◮He iti te tono tauira, me tango he kakano kotahi.

◮Kaore e hiahiatia nga maimoatanga motuhake penei i te huri me te kuru, a he ngawari te mahi.

◮Ko te punaha PCR kua arotauhia ka taea e te PCR te whai kiko ake me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

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Foreasy Taq DNA Polymerase

◮Te tino motuhake: He mahi whakaoho wera to te enzyme.

◮Whakanuia Tere: 10 sec/kb.

◮Te tauira tino urutau: ka taea te whakamahi ki te whakanui i te GC Te uara teitei, nga momo tauira tauira DNA uaua-ki-whakanui.

◮Tino pono: Taq Enzyme 6 nga wa.

◮Te kaha o te waiariki: Ka taea te whakanoho ki te 37 °C mo te wiki me te pupuri i nga mahi neke atu i te 90%.

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Foreasy HS Taq DNA Polymerase

◮Te tino motuhake: Ko te enzyme he nui te mahi wera wera.

◮Whakanuia Tere: 10 sec/kb.

◮Te urutau tauira teitei: ka taea te whakamahi kia pai te whakanui i te TeiteiGCuaraamomo tauira DNA uaua-ki-whakanui.

◮Te piripono kaha: Ko te pono e 6 nga waof noa Taq Enzyme.

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Kete PCR Tika Tiu Kararehe (kaore he Utauta Tauira) Kawa

Whakamahi tika i te lysate kikokiko kararehe hei tauira mo te whakakaha PCR, kaore he tangohanga motuhake o te DNA, he tere me te tairongo teitei.

◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui.

◮He iti te tono tauira, me tango he kakano kotahi.

◮Kaore e hiahiatia nga maimoatanga motuhake penei i te huri me te kuru, a he ngawari te mahi.

◮Ko te punaha PCR kua arotauhia ka taea e te PCR te whai kiko ake me te kaha ake o te manawanui ki nga aukati tauhohenga PCR.

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Wā Tuturu PCR Easyᵀᴹ-Taqman

◮Maama—2× PCR Mix hei whakaiti i te hapa whakamatautau me te wa mahi

◮Ka taea e te parepare kua tino arotautia me te wera-whana Taq enzyme te aukati i te whakakaha kore-motuhake me te hanga dimer tuatahi

◮Te tairongo teitei—ka kitea nga kape iti o te tauira

◮He pai te mahi—he hototahi ki te nuinga o nga taputapu PCR ine i te waa-tūturu

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RT Easyᵀᴹ II Master Premix mō te kōtuitanga cDNA whenu tuatahi mo PCR Wā Tūturu

- He pai te punaha tuhi whakamuri, 20 meneti noa te roa hei whakaoti i te whakahiatotanga o te whenu cDNA tuatahi.

- He punaha whakamuri whakamuri teitei, ka taea e nga tauira taumata-pg te tiki cDNA kounga teitei.

- Ko te punaha tuhi whakamuri he nui te waiariki, ko te pāmahana tauhohenga o te punaha ka eke ki te 50 ℃, he pai te mahi whakamuri.

- Ahakoa nga tauira RNA poke (te waipiro tae atu ki te 60%, te tote guanidine tae atu ki te 750mM) ka tukuna ki te tauhohenga whakawhiti whakamuri.

- 2× RT RĀNEI-Ngāwari^TM Kua taapirihia e te ranunga nga tuhinga tuatahi whakamuri whakamuri (Random Primer, Oligo(dT)₁₈ Primer).

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Toto SuperDirectᵀᴹ PCR Kit-EDTA Blood Direct PCR Master Mix mo te Genotyping o te Toto

Whakamahi tika i te EDTA anticoagulated toto katoa hei tauira mo te whakakaha PCR, he nui te tairongo me te kaha whakakaha teitei.

◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui, kaore he maimoatanga o mua, me te whakamahi tika i te toto katoa hei tauira PCR.

◮He kaha te kaha ki te whakakaha ake i te punaha, a ka taea e ia te kite i te ira ira me nga kongakonga DNA whainga kee i roto i te toto.

◮2× SuperEasy^TMWhakaranu-EDTA Te manawanui o te toto: tae atu ki te 45% i roto i te toto tangata me te 20% i te toto kiore.

◮Kua kapi katoa te tauira, no reira kaore he take e maaharahara mo te pokenga tauira me nga hua PCR pai.

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PCR Heroᵀᴹ (Me te Waikano)

◮Te pono teitei ake: e 6 nga wa i to Taq enzyme noa;

◮Tere whakakaha tere

◮He nui ake te urutau tauira

◮He pai ake te whakakaha ake

◮He kaha ake te manawanui o te taiao: ka whakanohoia ki te 37 ° C mo te wiki, me te pupuri i nga mahi neke atu i te 90%;

◮He 5'→3' te mahi polymerase DNA me te 5'→3' mahi exonuclease, kaore he mahi exonuclease 3'→5'.

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Toto SuperDirectᵀᴹ PCR Kit-Heparin Toto Direct PCR Master Mix mo te Genotyping o te Toto

Whakamahi tika i te heparin anticoagulated toto katoa hei tauira mo te whakakaha PCR, he nui te tairongo me te kaha o te whakakaha.

◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui, kaore he maimoatanga o mua, me te whakamahi tika i te toto katoa hei tauira PCR.

◮He kaha te kaha ki te whakakaha ake i te punaha, a ka taea e ia te kite i te ira ira me nga kongakonga DNA whainga kee i roto i te toto.

◮2× SuperEasy^TMWhakaranu-Heparin Whakaaetanga toto teitei: tae atu ki te 45% ki te toto tangata me te 20% ki te toto kiore.

◮Kua kapi katoa te tauira, no reira kaore he take e maaharahara mo te pokenga tauira me nga hua PCR pai.

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Toto SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Toto Direct PCR Master Mix mo te Genotyping o te Toto

Whakamahi tika i te heparin anticoagulated toto katoa hei tauira mo te whakakaha PCR, he nui te tairongo me te kaha o te whakakaha.Kua taapirihia te punaha anti-pollution UNG hei aukati i nga hua pai.

◮He kaha te kaha ki te whakakaha ake i te punaha, a ka taea e ia te kite i te ira ira me nga kongakonga DNA whainga kee i roto i te toto.

◮2× SuperEasy^TMWhakaranu(UNG)-Heparin He nui te manawanui o te toto: tae atu ki te 45% ki te toto tangata me te 20% ki te toto kiore.

◮Kua kapi katoa te tauira, no reira kaore he take e maaharahara mo te pokenga tauira me nga hua PCR pai.Pūnaha PCR anti-pollution

◮2×SuperEasy^TMWhakaranuhia (UNG), te whakakore i te parahanga na nga hua PCR, me te whakarite i te motuhake me te tika o te whakanui.

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Toto SuperDirectᵀᴹ PCR Kit(UNG)-EDTA Toto Direct PCR Master Mix mo te Whakatauira Toto

Whakamahi tika i te EDTA anticoagulated toto katoa hei tauira mo te whakakaha PCR, he nui te tairongo me te kaha whakakaha teitei.Kua taapirihia te punaha anti-pollution UNG hei aukati i nga hua pai.

◮Kaore e hiahiatia te purenga DNA e pau ana te wa me te utu nui, kaore he maimoatanga o mua, me te whakamahi tika i te toto katoa hei tauira PCR.

◮He kaha te kaha ki te whakakaha ake i te punaha, a ka taea e ia te kite i te ira ira me nga kongakonga DNA whainga kee i roto i te toto.

◮2× SuperEasy^TMKo te Mix(UNG)-EDTA he nui te manawanui o te toto: tae atu ki te 45% ki te toto tangata me te 20% ki te toto kiore.

◮Kua kapi katoa te tauira, no reira kaore he take e maaharahara mo te pokenga tauira me nga hua PCR pai.Pūnaha PCR anti-pollution

◮2×SuperEasy^TMWhakaranu (UNG) -EDTA, ka whakakore pai i te parahanga na nga hua PCR, me te whakarite i te motuhake me te tika o te whakanui.

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